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Serotonin axons of the ependyma and circumventricular organs in the forebrain of the guinea pig

An immunohistochemical study

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Summary

The distribution of supraependymal nerve fibers (SEF) containing serotonin (5-HT) was investigated immunohistochemically in the forebrain of the guinea pig. The highest densities of immunoreactive axons were found in the pars centralis and the inferior horn of the lateral ventricle and also in the superior part of the third ventricle. Because of the special development of the choroid plexus in these ventricular regions, it is suggested that 5-HT SEF might be involved in the regulation of the composition of the cerebrospinal fluid. The ependyma lining the circumventricular organs located in the forebrain, was not observed to receive a significant 5-HT-SEF innervation. In the pituitary gland, a loose but constant network of 5-HT axons, resembling those which course in the anterobasal hypothalamus, arcuate nucleus and internal layer of the median eminence, was observed in the neural lobe. In the epiphysis, immunoreactive 5-HT was detected in all pinealocytes (the entire cell was filled with reaction product) and in fibers running between them.

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References

  • Aghajanian GK, Gallager DW (1975) Raphe origin of serotonergic nerves terminating in the cerebral ventricles. Brain Res 88:221–231

    Google Scholar 

  • Aghajanian GK, Bloom FE, Lovell RA, Sheard MH, Freedman DX (1966) The uptake of 5-hydroxytryptamine3H from the cerebral ventricles: autoradiographic localization. Biochem Pharmacol 15:1401–1403

    Google Scholar 

  • Alonso G, Pons F, Cadilhac J (1974) Mise en évidence par radioautographie de terminaisons indolaminergiques dans les parois ventriculaires cérébrales chez le Rat. C R Soc Biol 168:1021–1024

    Google Scholar 

  • Andén NE, Dahlström A, Fuxe K, Larsson K (1965) Mapping out of catecholamine and 5-hydroxytryptamine neurons innervating the telencephalon and diencephalon. Life Sci 4:1275–1279

    Google Scholar 

  • Arluison M, Bouchaud C, Derer P, Di Marco C (1976) Sur la présence de terminaisons sus-épendymaires “géantes” dans le cerveau des rongeurs. C R Acad Sci Paris 282:381–383

    Google Scholar 

  • Bouchaud C, Arluison M (1977) Serotoninergic innervation of ependymal cells in the rat subcommissural organ. A fluorescence, electron microscopic and radioautographic study. Biol Cellulaire 30:61–64

    Google Scholar 

  • Chan-Palay V (1976) Serotonin axons in the supra- and subependymal plexuses and in the leptomeninges; their roles in local alterations of cerebrospinal fluid and vasomotor activity. Brain Res 102:103–130

    Google Scholar 

  • Chan-Palay V (1977) Indoleamine neurons and their processes in the normal rat brain and in chronic diet-induced thiamine deficiency demonstrated by uptake of 3H-serotonin. J Comp Neurol 176:467–494

    Google Scholar 

  • Dahlström A, Fuxe K (1964) Evidence for the existence of monoamine containing neurons in the central nervous system. I. Demonstration of monoamines in cell bodies of brain stem neurons. Acta Physiol Scand 62:1–55

    Google Scholar 

  • Feldberg W, Myers RD (1966) Appearance of 5-hydroxytryptamine and an unidentified pharmacologically active lipid acid in effluent from perfused cerebral ventricles. J Physiol (London) 184:837–855

    Google Scholar 

  • Fuxe K, Jonsson G (1974) Further mapping of central 5-hydroxytryptamine neurons: Studies with the neurotoxic dihydroxytryptamines. In: Costa E, Gessa GL, Sander M (eds) Advances in biochemical psychopharmacology, Vol 10. Raven Press, New York, pp 1–12

    Google Scholar 

  • Fuxe K, Ungerstedt U (1968) Histochemical studies on the distribution of catecholamines and 5-hydroxytryptamine after intraventricular injections. Histochemie 13:16–28

    Google Scholar 

  • Juillard MT, Collin JP (1980) Pools of serotonin in the pineal gland of the mouse: the mammalian pinealocyte as a component of the diffuse neuroendocrine system. Cell Tissue Res 213:273–291

    Google Scholar 

  • Köhler C, Chan-Palay V, Steinbusch H (1981) The distribution and orientation of serotonin fibers in the entorhinal and other retrohippocampal areas. An immunohistochemical study with anti-serotonin antibodies in the rat's brain. Anat Embryol 161:237–264

    Google Scholar 

  • Kordon C, Héry M, Szafarczyk A, Ixart G, Assenmacher I (1981) Serotonin and the regulation of pituitary hormone secretion and of the neuroendocrine rhythms. J Physiol Paris 77:489–496

    Google Scholar 

  • Le Beux YJ (1972) An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland. II Nerve endings. Z Zellforsch 127:439–461

    Google Scholar 

  • Leonhardt H, Lindner E (1967) Marklose Nervenfasern im III. und IV. Ventrikel des Kaninchen- und Katzengehirns. Z Zellforsch 78:1–18

    Google Scholar 

  • Lidov HGW, Grzanna R, Molliver ME (1980) The serotonin innervation of the cerebral cortex in the rat — an immunohistochemical analysis. Neuroscience 5:207–227

    Google Scholar 

  • Lorez HP (1976) Do supra-ependymal serotoninergic (5-HT) nerves regulate ciliary activity in the rat brain? Experientia 32:802

    Google Scholar 

  • Lorez HP, Richards JG (1973) Distribution of indolealkylamine nerve terminals in the ventricles of the rat brain. Z Zellforsch 144:511–522

    Google Scholar 

  • Lorez HP, Richards JG (1975) 5-HT nerve terminals in the fourth ventricle of the rat brain: their identification and distribution studied by fluorescence histochemistry and electron microscopy. Cell Tissue Res 165:37–48

    Google Scholar 

  • Olivereau M, Olivereau JM, Aimar C (1980) Responses of MSH and prolactin cells to 5-hydroxytryptophan (5-HTP) in amphibians and teleosts. Cell Tissue Res 207:377–385

    Google Scholar 

  • Palkovits M, Saavedra JM, Jacobowitz DM, Kizer JS, Zaborszky L, Brownstein MJ (1977) Serotonergic innervation of the forebrain: effect of lesions on serotonin and tryptophan hydroxylase levels. Brain Res 130:121–134

    Google Scholar 

  • Parent A, Descarries L, Beaudet A (1981) Organization of ascending serotonin systems in the adult rat brain. A radioautographic study after intraventricular administration of (3H)5-hydroxytryptamine. Neuroscience 6:115–138

    Google Scholar 

  • Ribas JL (1977) Morphological evidence for a possible functional role of supraependymal nerves on ependyma. Brain Res 125:362–368

    Google Scholar 

  • Richards JG (1977) Autoradiographic evidence for the selective accumulation of (3H) 5-HT by supraependymal nerve terminals. Brain Res 134:151–157

    Google Scholar 

  • Richards JG, Lorez HP, Tranzer JP (1973) Indolealkylamine nerve terminals in cerebral ventricles: identification by electron microscopy and fluorescence histochemistry. Brain Res 57:277–288

    Google Scholar 

  • Richards JG, Lorez HP, Colombo VE, Guggenheim R, Kiss D (1980) Supra-ependymal nerve fibres in human brain: correlative transmission and scanning electron microscopical and fluorescence histochemical studies. Neuroscience 5:1489–1502

    Google Scholar 

  • Richards JG, Lorez HP, Colombo VE, Guggenheim R, Kiss D, Wu JY (1981) Demonstration of supraependymal 5-HT nerve fibres in human brain and their immunohistochemical identification in rat brain. J Physiol Paris 77:219–224

    Google Scholar 

  • Sapun DI, Farah JM, Mueller GP (1981) Evidence that a serotoninergic mechanism stimulates the secretion of pituitary β-endorphin-like immunoreactivity in the rat. Endocrinology 109:421–426

    Google Scholar 

  • Stefanini M, De Martino C, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173

    Google Scholar 

  • Steinbusch HWM (1981) Distribution of serotonin immunoreactivity in the central nervous system of the rat. Cell bodies and terminals. Neuroscience 6:557–618

    Google Scholar 

  • Steinbusch HWM, Verhofstad AAJ, Joosten HWJ (1978) Localization of serotonin in the central nervous system by immunohistochemistry: description of a specific and sensitive technique and some applications. Neuroscience 3:811–819

    Google Scholar 

  • Takeuchi Y, Kimura H, Sano Y (1982) Immunohistochemical demonstration of the distribution of serotonin neurons in the brainstem of the rat and cat. Cell Tissue Res 224:247–267

    Google Scholar 

  • Ternaux JP, Boireau A, Bourgoin S, Hamon M, Héry F, Glowinski J (1976) In vivo release of 5-HT in the lateral ventricle of the rat: effects of 5-hydroxytryptophan and tryptophan. Brain Res 101:533–548

    Google Scholar 

  • Tochino Y, Schanker LS (1965) Transport of serotonin and norepinephrine by the rabbit choroid plexus in vitro. Biochem Pharmacol 14:1557–1566

    Google Scholar 

  • Tramu G, Léonardelli J (1979) Immunohistochemical localization of enkephalin in median eminence and adenohypophysis. Brain Res 168:457–471

    Google Scholar 

  • Tramu G, Beauvillain JC, Croix D, Léonardelli J (1981) Comparative immunocytochemical localization of enkephalin and somatostatin in the median eminence, hypothalamus and adjacent areas of the guinea-pig brain. Brain Res 215:235–257

    Google Scholar 

  • Westergaard E (1972) The fine structure of nerve fibers and endings in the lateral cerebral ventricles of the rat. J Comp Neurol 144:345–354

    Google Scholar 

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Authors and Affiliations

  1. U. 156 INSERM, Lille, France

    G. Tramu, A. Pillez & J. Leonardelli

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  1. G. Tramu
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  2. A. Pillez
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  3. J. Leonardelli
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Tramu, G., Pillez, A. & Leonardelli, J. Serotonin axons of the ependyma and circumventricular organs in the forebrain of the guinea pig. Cell Tissue Res. 228, 297–311 (1983). https://doi.org/10.1007/BF00204880

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  • DOI: https://doi.org/10.1007/BF00204880

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