Abstract
In Brassica, self-incompatibility has been correlated with S-locus glycoproteins (SLG) localized at the site of pollen rejection, the stigmatic papillae. In this paper we present immunocytochemical evidence that during development of the pistil SLG proteins accumulate not only in the stigmatic papillae, but also in the transmitting tissue of stigma, style and ovary, along the pathway followed by the pollen tube. This observation was confirmed by the biochemical characterization of stylar and ovarian SLG proteins and by the detection of SLG transcripts in style/ovary tissues by polymerase chain reaction analysis. Thus we demonstrate similarities between the pattern of SLG gene expression in Brassica (sporophytic self-incompatibility system) and that of S products in Nicotiana (gametophytic self-incompatibility system) which may reflect relationships between the self-incompatibility systems. An active role of SLG gene products during the pollen-tube growth in the pistil is discussed.
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Abbreviations
- IEF:
-
isoelectric focusing
- Mab:
-
monoclonal antibody
- PCR:
-
polymerase chain reaction
- SLG:
-
S-locus glycoprotein
- SLG-Sc:
-
self-compatible S-locus glycoprotein
- SLR:
-
S-locus related
- SRK:
-
S-locus receptor kinase
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We would like to thank Richard Blanc and Monique Estienne for technical assistance; Pierre Audenis for photographic work. T.G. and M.R. are members of the Centre National de la Recherche Scientifique, M.C. is member of the Institut National de la Recherche Agronomique.
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Kleman-Mariac, C., Rougier, M., Cock, J.M. et al. S-locus glycoproteins are expressed along the path of pollen tubes in Brassica pistils. Planta 196, 614–621 (1995). https://doi.org/10.1007/BF00203663
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DOI: https://doi.org/10.1007/BF00203663