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Inhibition of the synthesis of cell wall polysaccharides in oat coleoptile segments by jasmonic acid: Relevance to its growth inhibition

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Abstract

The inhibitory mode of action of jasmonic acid (JA) on the growth of etiolated oat (Avena sativa L. cv. Victory) coleoptile segments was studied in relation to the synthesis of cell wall polysaccharides using [14C]glucose. Exogenously applied JA significantly inhibited indoleacetic acid (IAA)-induced elongation of oat coleoptile segments and prevented the increase of the total amounts of cell wall polysaccharides in both the noncellulosic and cellulosic fractions during coleoptile growth. JA had no effect on neutral sugar compositions of hemicellulosic polysaccharides but substantially inhibited the IAA-stimulated incorporation of [14C]glucose into noncellulosic and cellulosic polysaccharides. JA-induced inhibition of growth was completely prevented by pretreating segments with 30 mm sucrose for 4 h before the addition of IAA. The endogenous levels of UDP-sugars, which are key intermediates for the synthesis of cell wall polysaccharides, were not reduced significantly by JA. Although these observations suggest that the inhibitory mode of action of JA associated with the growth of oat coleoptile segments is relevant to sugar metabolism during cell wall polysaccharide synthesis, the precise site of inhibition remains to be investigated.

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Abbreviations

JA:

jasmonic acid

ABA:

abscisic acid

IAA:

indoleacetic acid

T 0 :

minimum stress relaxation time

TFA:

trifluoroacetic acid

TCA:

trichloroacetic acid

HPLC:

high-performance liquid chromatography

EtOAc:

ethyl acetate

TLC:

thin-layer chromatography

JA-Me:

methyl jasmonate

GLC-SIM:

gas-liquid chromatography-selected ion monitoring

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Authors and Affiliations

  1. College of Integrated Arts and Sciences, University of Osaka Prefecture, Gakuen-cho, 593, Sakai-city, Osaka, Japan

    Junichi Ueda & Kensuke Miyamoto

  2. Faculty of Science, Osaka City University, Sugimoto, 665, Osaka, Japan

    Seiichiro Kamisaka

Authors
  1. Junichi Ueda
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  2. Kensuke Miyamoto
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  3. Seiichiro Kamisaka
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Ueda, J., Miyamoto, K. & Kamisaka, S. Inhibition of the synthesis of cell wall polysaccharides in oat coleoptile segments by jasmonic acid: Relevance to its growth inhibition. J Plant Growth Regul 14, 69–76 (1995). https://doi.org/10.1007/BF00203116

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  • DOI: https://doi.org/10.1007/BF00203116

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