Skip to main content
SpringerLink
Log in

In vitro binding of Campylobacter jejuni surface proteins to murine small intestinal cell membranes

  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

Surface proteins of nine Campylobacter jejuni strains belonging to three different serovia were extracted with lysozyme/ethylenediamine-tetraacetic acid. The preparations bound to isolated murine small intestinal cells and to a membrane fraction (MF) of isolated brush borders obtained by detergent treatment with Triton X-100 and Nonidet P40. Binding was demonstrated by an enzyme-linked immunosorbent assay procedure. Using lipopolysaccharide (LPS)- and flagella-specific antisera the contribution of flagella and LPS, present in the protein preparations, to the total binding to MF was investigated. Only up to approximately 10% of the total binding of each strain was found to be mediated by LPS, 10%–33% of binding was flagella dependent. The preparations of four strains (serovar HS2) bound in a trypsin-sensitive manner (45%–85% reduction), while the others (serovaria HS1 and HS13) were hardly influenced by trypsin treatment. Binding to MF was not impaired by preincubation of the bacterial surface protein preparations with several sugars and lectins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bätza HJ, Rübsamen S, Schliesser T (1983) Zum Vorkommen von Campylobacter fetus subsp. jejuni bei Mäusen und Meerschweinchen aus Versuchstierhaltungen. Zentralbl Veterinarmed [B] 30:455–461

    Google Scholar 

  2. Beachy EH (ed) (1980) Bacterial adherence, receptors and recognition: series B, vol 6. Chapman and Hall, New York, pp 187–245

    Google Scholar 

  3. Blaser MJ, Laforce FM, Wilson NA, Wang WL (1980) Reservoirs for human Campylobacteriosis. J Infect Dis 141:665–669

    Google Scholar 

  4. Blaser MJ, Hopkins JA, Berka RM, Vasil ML, and Wang WL (1983) Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun 42:276–284

    Google Scholar 

  5. Blaser MJ, Hopkins JA, Vasil ML (1984) Campylobacter jejuni outer membrane proteins are antigenic for humans. Infect Immun 43:986–993

    Google Scholar 

  6. Blaser MJ, Hopkins JA, Perez-Perez GI, Cody HJ, Newell DG (1986) Antigenicity of Campylobacter jejuni flagella. Infect Immun 53:47–52

    Google Scholar 

  7. Cinco M, Banfi E, Ruaro E, Crevatin D, Crotti D (1984) Evidence for l-fucose (6-deoxy-l-galactopyranose)-mediated adherence of Campylobacter spp. to epithelial cells. FEMS Microbiol Lett 21:347–351

    Google Scholar 

  8. Engvall E, Perlman P (1972) Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. J Immunol 109:129–135

    Google Scholar 

  9. Fauchere JL, Rosenau A, Vernon M, Moyen EN, Richard S, Pfister A (1986) Association with Hela cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. Infect Immun 54:283–287

    Google Scholar 

  10. Feter R, O'Brian PCM (1981) Role of chemotaxis in the association of motile bacteria with intestinal mucosa: fitness and virulence of nonchemotactic Vibrio cholerae mutants in infant mice. Infect Immun 34:222–233

    Google Scholar 

  11. Kaijser B (1988) Campylobacter jejuni/coli. APMIS 96:283–288

    Google Scholar 

  12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    PubMed  Google Scholar 

  13. Laux DC, McSweegan E, Williams T, Wadolkowski E, Cohen P (1986) Identification and characterization of mouse intestinal mucosal receptors for Escherichia coli K-12(K88ab). Infect Immun 52:18–25

    CAS  PubMed  Google Scholar 

  14. Lee A, O'Rourke JL, Barrington PJ, Trust TJ (1986) Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse cecal model. Infect Immun 51:536–546

    Google Scholar 

  15. Lee A, Logan SM, Trust TJ (1987) Demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus. Infect Immun 55:828–831

    Google Scholar 

  16. Lior H, Woodward DL, Edgar JA, Laroche LJ, Gill P (1982) Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol 15:761–768

    Google Scholar 

  17. Logan SM, Trust TJ (1982) Outer membrane characteristics of Campylobacter jejuni. Infect Immun 38:898–906

    Google Scholar 

  18. Logan SM, Trust TJ (1983) Molecular identification of surface antigens of Campylobacter jejuni. Infect Immun 42:675–682

    Google Scholar 

  19. Logan SM, Trust TJ (1984) Structural and antigenic heterogeneity of lipopolysaccharides of Campylobacter jejuni and Campylobacter coli. Infect Immun 45:210–216

    Google Scholar 

  20. Lowry OH, Rosenbrough N, Farr A, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  21. Manninen KI, Prescott JF, Dohoo IR (1982) Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infect Immun 38:46–52

    Google Scholar 

  22. McSweegan E, Walker RI (1986) Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun 53:141–148

    Google Scholar 

  23. Mills SD, Bradbury WC (1984) Human antibody response to outer membrane proteins of Campylobacter jejuni during infection. Infect Immun 43:739–749

    Google Scholar 

  24. Mizushima S, Yamada H (1975) Isolation and characterization of two outer membrane preparation from Escherichia coli. Biochim Biophys Acta 365:44–53

    Google Scholar 

  25. Morooka T, Umeda A, Amako K (1985) Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol 131:1973–1980

    Google Scholar 

  26. Nachamkin I, Hart AM (1985) Western blot analysis of the human antibody response to Campylobacter jejuni during gastrointestinal infection. Infect Immun 21:33–38

    Google Scholar 

  27. Newell DB, Pearson AD (1982) Pathogenicity of Campylobacter jejuni an in vitro model of adhesion and invasion? In: Newell DG (ed) Campylobacter epidemiology, pathogenesis and biochemistry. MTP Press, Lancaster, pp 196–199

    Google Scholar 

  28. Newell DG, McBride H (1985) Investigations on the role of flagella in the colonization of infant mice with Campylobacter jejuni and attachment of Campylobacter jejuni to human epithelial cell lines. J Hyg Camb 95:217–227

    Google Scholar 

  29. Newell DG (1986) Monoclonal antibodies directed against the flagella of Campylobacter jejuni: Production, characterization and lack of effect on the colonization of infant mice. J Hyg (Lond) 96:131–141

    Google Scholar 

  30. Penner JL, Hennessy JN (1980) Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol 12:732–737

    Google Scholar 

  31. Sachs L (1969) Angewandte Statistik, 2nd edn. Springer, Berlin Heidelberg New York, p 342

    Google Scholar 

  32. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    CAS  PubMed  Google Scholar 

  33. Treschnak E, Moser I, Hellmann E (1987) Bestimmung der Spezies, des Plasmidmusters und der Chemotherapeutika-Resistenz von aus Darmausscheidungen von Haustieren und Menschen isolierten Campylobacter-Stämmen. Tierärztl Umsch 42:133–144

    Google Scholar 

  34. Wenman WM, Chai J, Louie TJ, Goudreau C, Lior H, Newell DG, Pearson AD, Taylor DE (1985) Antigenic analysis of Campylobacter flagellar protein and other proteins. J. Clin Microbiol 21:108–112

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie und Tierhygiene, Fachbereich Veterinärmedizin, Freie Universität Berlin, Koserstrasse 20, D-1000, Berlin 33

    I. Moser & E. Hellmann

Authors
  1. I. Moser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Hellmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the Deutsche Forschungsgemeinschaft (Mo 425/1-1 and 2)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moser, I., Hellmann, E. In vitro binding of Campylobacter jejuni surface proteins to murine small intestinal cell membranes. Med Microbiol Immunol 178, 217–228 (1989). https://doi.org/10.1007/BF00202555

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00202555

Keywords

Search

Navigation