Abstract
The composition and phase behavior of some lipid classes and mixtures of thylakoid polar lipids were measured to investigate their role as determinants of the temperature of the transition associated with chilling injury. For Nerium oleander L., a plant which acclimates to growth temperature, a mixture of the phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG) showed transition temperatures of 22° and 10° C for plants grown at 45° and 20° C, respectively. This difference was similar to the 9 Celsius degrees differential in the transition of the polar lipids and indicated that the PG and-or the PG-SQDG mixture could be the major determinants of the transition temperature. Reconstitution of the PG-SQDG mixture from 20°-grown oleander with the galactolipids from 45°-grown plants, however, reduced the transition temperature by only 4 Celsius degrees. This indicates that some, low-melting-point lipids, which are structurally capable of forming a co-gel with the high-melting-point lipids, also play a role in determining the temperature of the transition and that the composition of these low-melting-point lipids also changes with growth temperature. More specific information on the role of PG was obtained using polar lipids from Cucumis sativus L., a chilling-sensitive plant. For this material the transition in the polar lipids was reduced from 9° to 5° and 4° C when the transition of the PG was reduced from 32° to 25° and 22° C. This was accomplished by reducing the proportion of disaturated molecular species in PG from 78 to 56 and 44 mol% by the addition of a fraction of the PG enriched in unsaturated molecular species. The data indicate that the transition temperature of the polar lipids of cucumber would be reduced to below 0° C, typical of a chillinginsensitive plant, when the transition temperature of PG was reduced to 15° C and this would occur at 21 mol% of disaturated molecular species. It is concluded that the transition in the thylakoid polar lipids, associated with chilling injury, involves both high- and low-meltingpoint lipids but can be reduced when the transition temperature of the high-melting-point component is reduced.
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Abbreviations
- DGDG:
-
digalactosyldiacylglycerol
- MGDG:
-
monogalactosyldiacylglycerol
- PG:
-
phosphatidylglycerol
- SQDG:
-
sulfoquinovosyldiacylglycerol
References
Allen, C.F., Good, P. (1971) Acyl lipids in photosynthetic systems. Methods Enzymol. 23, 523–547
Ames, B.N. (1966) Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol. 8, 115–118
Anderson, J.M., Boardman, N.K., Spencer, D. (1972) Phosphorylation by intact bundle sheath chloroplasts from maize. Biochim. Biophys. Acta 245, 253–258
Bishop, D.G. (1987) Analysis of molecular species of chloroplast lipids by HPLC. J. Liquid Chromatogr. 10, 1497–1505
Bishop, D.G., Kenrick, J.R. (1987) Thermal properties of 1-hexadecanoyl, 2-trans-3,hexadecenoyl phosphtidylglycerol. Phytochemistry 26, 3065–3067
Demandre, C., Tremolieres, A., Justin, A., Mazliak, P. (1985) Analysis of molecular species of plant polar lipids by high performance and gas liquid chromatography. Phytochemistry 24, 481–485
Fork, D.C., van Ginkel, G., Harvey, G. (1981) Phase transition temperatures determined for chloroplast thylakoid membranes and liposomes prepared from charged lipids extracted from thylakoids of higher plants. Plant Cell Physiol. 22, 1035–1042
Frentzen, M., Heinz, E., McKeon, T.A., Stumpf, P.K. (1983) Specificities and selectivities of glycerol-3-phosphate acyltransferase and monoacylglycerol-3-phosphate acyltransferase from pea and spinach chloroplasts. Eur. J. Biochem. 129, 629–636
Frentzen, M., Nishida, I., Murata, N. (1987) Properties of Plastidal acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase from the chilling-sensitive plant squash (Cucurbita moschata). Plant Cell Physiol. 28, 1195–1201
Hodgson, R.A.J., Orr, G.R., Raison, J.K. (1987) Inhibition of photosynthesis by chilling in the light. Plant Sci. 49, 75–79
Kenrick, J.R., Bishop, D.G. (1986) The fatty acid composition of phosphatidylglycerol and sulfoquinovosyldiacylglycerol of higher plants in relation to chilling sensitivity. Plant Physiol. 81, 946–949
Lyons, J.M. (1973) Chilling injury in plants. Annu. Rev. Plant Physiol. 24, 455–466
Lyons, J.M., Raison, J.K. (1970) Oxidative activity of mitochondria isolated from plant tissue sensitive and resistant to chilling injury. Plant Physiol. 45, 386–389
Murata, N. (1983) Molecular species composition of phosphatidylglycerols from chilling-sensitive and chilling resistant plants. Plant Cell Physiol. 24, 81–86
Murata, N., Yamaya, J. (1984) Temperature-dependent phase behavior of phosphatidylglycerols from chilling-sensitive and chilling-resistant plants. Plant Physiol. 74, 1016–1024
Nishida, I., Frentzen, M., Ishizaki, O., Murata, N. (1987) Purification of isomeric forms of acyl-[acyl-carrier-protein]:glycerol-3-phosphate acyltransferase from greening squash cotyledons. Plant Cell Physiol. 28, 1071–1079
Orr, G.R., Raison, J.K. (1987) Compositional and thermal properties of thylakoid polar lipids of Nerium oleander L. in relation to chilling sensitivity. Plant Physiol. 84, 88–92
Pollard, J.H. (1977) A handbook of numerical and statistical techniques. Cambridge University Press, Cambridge, UK
Pike, C.S., Berry, J.A. (1980) Membrane phospholipid phase separations in plants adapted to or acclimated to different thermal regions. Plant Physiol. 66, 238–241
Raison, J.K., Chapman, E.A. (1976) Membrane phase changes in chilling-sensitive Vigna radiata and their significance to growth. Aust. J. Plant Physiol. 3, 291–299
Raison, J.K., Orr, G.R. (1986) Phase transitions in the thylakoid polar lipids of chilling-sensitive plants: a comparison of detection methods. Plant Physiol. 80, 638–645
Raison, J.K., Wright, L.C. (1983) Thermal phase transitions in the polar lipids of plant membranes: their induction by disaturated phospholipids and their possible relation to chilling injury. `Biochim. Biophys. Acta 731, 69–78
Raison, J.K., Chapman, E.A., Wright, L.C., Jacobs, S.W.L. (1979) Membrane lipid transitions: their correlation with climatic distribution of plants. In: Low temperature stress in crop plants: the role of the membrane, pp 177–186. Lyons, J.M., Graham, D.G., Raison, J.K., eds. Academic Press, New York
Raison, J.K., Pike, C.S., Berry, J.A. (1982) Growth temperature-induced alterations in the thermotropic properties of Nerium oleander membrane lipids. Plant Physiol. 70, 215–218
Roughan, P.G. (1985) Phosphatidylglycerol and chilling sensitivity in plants. Plant Physiol. 77, 740–746
Roughan, P.G., Batt, R.D. (1968) Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues. Anal. Biochem. 22, 74–88
Shipley, G.G., Green, J.P., Nichols, B.W. (1973) The phase behavior of monogalactosyl, digalactosyl and sulphoquinovosyl diglycerides. Biochim. Biophys. Acta 341, 531–544
Shneyour, A., Raison, J.K., Smillie, R.M. (1973) The effect of temperature on the rate of photosynthetic electron transfer in chloroplasts of chilling-sensitive and chilling-resistant plants. Biochim Biophys. Acta 292, 152–161
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Orr, G.R., Raison, J.K. The effect of changing the composition of phosphatidylglycerol from thylakoid polar lipids of oleander and cucumber on the temperature of the transition related to chilling injury. Planta 181, 137–143 (1990). https://doi.org/10.1007/BF00202336
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DOI: https://doi.org/10.1007/BF00202336