Abstract
The safener-induced maize (Zea mays L.) glutathione S-transferase, GST II (EC 2.5.1.18) and another predominant isoform, GST I, were purified from extracts of maize roots treated with the safeners R-25788 (N,N-diallyl-2-dichloroacetamide) or R-29148 (3-dichloroace-tyl-2,2,5-trimethyl-1,3-oxazolidone). The isoforms GST I and GST II are respectively a homodimer of 29-kDa (GST-29) subunits and a heterodimer of 29 and 27-kDa (GST-27) subunits, while GST I is twice as active with 1-chloro-2,4-dinitrobenzene as GST II, GST II is about seven times more active against the herbicide, alachlor. Western blotting using antisera raised against GST-29 and GST-27 showed that GST-29 is present throughout the maize plant prior to safener treatment. In contrast, GST-27 is only present in roots of untreated plants but is induced in all the major aerial organs of maize after root-drenching with safener. The amino-acid sequences of proteolytic fragments of GST-27 show that it is related to GST-29 and identical to the 27-kDa subunit of GST IV.
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Abbreviations
- CDNB:
-
1-chloro-2,4-dinitrobenzene
- DEAE:
-
di-ethylaminoethyl
- FPLC:
-
fast protein liquid chromatography
- GSH:
-
reduced glutathione
- GST:
-
glutathione S-transferase
- GST-26:
-
26-kDa subunit of maize GST
- GST-27:
-
27-kDa subunit of maize GST
- GST-29:
-
29-kDa subunit of maize GST
- R-25788:
-
safener N,N-diallyl-2-dichloroacetamide
- R-29148:
-
safener 3-dichloroacetyl-2,2,5-trimethyl-1,3-oxazolidone
- RPLC:
-
reverse phase liquid chromatography
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We are grateful to M-M. Lay, ZENECA AG Products (formerly ICI Americas), Richmond, Calif., USA for providing [14C] R-25788. ZENECA Seeds in the UK is part of ZENECA Limited.
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Holt, D.C., Lay, V.J., Clarke, E.D. et al. Characterization of the safener-induced glutathione S-transferase isoform II from maize. Planta 196, 295–302 (1995). https://doi.org/10.1007/BF00201388
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DOI: https://doi.org/10.1007/BF00201388