Summary
Antigenic properties of bloodstains of human and non-human primates as well as other animal bloodstains were investigated by the inhibition ELISA using commercially available anti-human albumin (Alb), α2-macroglobulin (α2-M), fibrinogen, transferrin, and immunoglobulin G. In general, chimpanzee bloodstains showed strong cross-reactions with these antisera, and the extent of the cross-reactions of other animal bloodstains decreased largely with the phylogenic order, i.e., agile gibbon (ape), Old World monkeys (Japanese monkey and hamadryas baboon), New World monkeys (night monkey and tufted capuchin monkey), prosimians (grand galago and ring-tailed lemur) and other animals (rat, cattle, swine, goat, dog, cat, and chicken). Among these antisera, anti-human α2-M showed the weakest cross-reaction with chimpanzee bloodstains, and anti-human Alb showed next.
Zusammenfassung
Die Antigeneigenschaften von Blutspuren menschlicher und nichtmenschlicher Primaten und anderer Tiere wurden mit Hilfe des Inhibitions-ELISA unter Benutzung käuflich erhältlicher Anti-Human-Albumin-und Anti-Alpha2-makroglobulin-, Fibrinogen-, Transferrin- und IGG-Seren untersucht. Allgemein kann festgestellt werden, daß Blutspuren von Schimpansen die stärksten Kreuzreaktionen mit diesen Antiseren aufwiesen und daß das Ausmaß der Kreuzreaktionen von Blutspuren anderer Tiere deutlich in Verbindung mit dem phylogenetischen Rang, wie folgt, abnahm: agiler Gibbon, Hundsaffen (Japanischer Affe, Mantelpavian), Breitnasenaffen (Nachtaffen und Kapuziner), Halbaffen (Groß-Galago und Katta) und andere Tiere (Ratte, Rind, Schwein, Ziege, Hund, Katze und Huhn). Unter diesen Antiseren zeigten Anti-Human-Alpha2-Makroglobulin-Seren die schwächsten Kreuzreaktionen mit Blutspuren von Schimpansen; Anti-HumanAlbumin folgte hiernach.
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Supported in part by the Cooperation Research Program of the Primate Research Institute
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Tsutsumi, H., Htay, H.H., Sato, K. et al. Antigenic properties of human and animal bloodstains studied by enzyme-linked immunosorbent assay (ELISA) using various antisera against specific plasma proteins. Z Rechtsmed 99, 191–196 (1987). https://doi.org/10.1007/BF00201250
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DOI: https://doi.org/10.1007/BF00201250