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Determination and identification of sympathomimetic amines in blood samples from drivers by a combination of gas chromatography and mass spectrometry

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Summary

Gas chromatographic and mass spectrometric data concerning the analysis for certain sympathomimetic amines in blood samples from drivers are presented. In all cases where gas chromatography gave peaks at the correct retention times for amphetamine or phenmetrazine respectively, mass spectrometry confirmed the presence of the corresponding drugs in the extracts from blood. Drug-free blood samples gave neither gas chromatographic nor mass spectrometric responses at the retention times characteristic for amphetamine or phenmetrazine.

Computer-drawn mass spectra of phenmetrazine are shown in Fig. 2. Analytical data obtained from twelve drivers and one hospital patient are collected in the table.

Zusammenfassung

Blutproben von Kraftfahrern, die sympathomimetische aromatische Amine zu sich genommen hatten, zeigten gaschromatographische „Zacken“ mit den Retentionszeiten von Amphetamin oder Phenmetrazin (Preludin®). Eine Reihe solcher Fälle wurden mit Hilfe einer kombinierten gaschromatographisch-massenspektrometrischen Methodik untersucht. In sämtlichen Fällen konnte die vermutete Identität der beiden Amine massenspektrometrisch einwandfrei bestätigt werden. Blutproben von drogenfreien Personen, auf exakt gleiche Weise untersucht, gaben weder im Gaschromatogramm noch im Massenspektrum irgendwelchen Ausschlag.

Massenspektren von Phenmetrazin, rein und nach Isolierung aus Blutproben, wurden durch einen Computer berechnet und gezeichnet. Abb. 2 zeigt die Resultate.

Quantitative Analysen und einwandfreie Identifizierung durch Gaschromatographie und Massenspektroskopie sind noch bei Mengen von ca. 1 μg Amphetamin oder Phenmetrazin in Blutproben ausführbar.

Qualitative und quantitative Resultate der Untersuchung von 12 Kraftfahrern und einem Patienten sind in der Tabelle zusammengestellt.

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Authors and Affiliations

  1. Government Laboratory for Forensic Chemistry, Stockholm 60, Sweden

    R. Bonnichsen, A. C. Maehly, Y. M»rde, R. Ryhage & B. Schubert

  2. Laboratory for Mass Spectrometry, Karolinska Institutet, Stockholm 60, Sweden

    R. Bonnichsen, A. C. Maehly, Y. M»rde, R. Ryhage & B. Schubert

Authors
  1. R. Bonnichsen
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  2. A. C. Maehly
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  3. Y. M»rde
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  4. R. Ryhage
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  5. B. Schubert
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Bonnichsen, R., Maehly, A.C., M»rde, Y. et al. Determination and identification of sympathomimetic amines in blood samples from drivers by a combination of gas chromatography and mass spectrometry. Z Rechtsmed 67, 19–26 (1970). https://doi.org/10.1007/BF00200423

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