Summary
An in vitro system to measure nonspecific augmentation of cytotoxicity toward human melanoma cell lines is described. Mononuclear cells separated from peripheral blood on isopaque-ficoll gradients were cultured with the mitogens phytohemagglutinin, concanavalin-A, and pokeweed, with Bacillus Calmette-Guerin (BCG), or with allogeneic lymphocytes. In each case, marked augmentation of cytotoxicity toward both melanoma cell lines and a normal fibroblast line was observed. Populations enriched for T cells by E-rosetting or nylon fiber filtration showed unimpaired responsiveness to BCG, while populations depleted of T cells by E-rosetting showed neither “natural” cytotoxicity nor augmentation of cytotoxicity by BCG.
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Coates, A.S., Crawford, M. Cell mediated cytotoxicity to human melanoma cell lines: Nonspecific active in vitro augmentation. Cancer Immunol Immunother 3, 131–136 (1977). https://doi.org/10.1007/BF00200071
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DOI: https://doi.org/10.1007/BF00200071