Skip to main content

Advertisement

SpringerLink
Log in

Species-specific TNF induction of thymocyte proliferation

  • Original Articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Recombinant murine (rMu) tumor necrosis factor (TNF), in a standard comitogenic assay with phytohemagglutinin, induced murine thymocyte proliferation, while up to 10,000-fold higher concentrations of recombinant human TNF did not. The induction of thymocyte proliferation was dependent upon TNF concentration in a biphasic manner. Thus, 100 to 1000 units/ml TNF were near optimal while concentrations ≥1,000 units/ml caused apparent down regulation. The effect was abrogated by neutralizing antibody to rMu-TNF but not by neutralizing antibody to rMu-interleukin 1α or β. The rMu-TNF did not induce proliferation of the mature murine T-helper cell line, D10.G4.1, in the presence of mitogen. Taken together the results indicate that TNF, in a strictly species-specific manner, can regulate thymocyte proliferation independently of interleukin 1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B (1975) An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA 72:3666–3670

    CAS  PubMed  Google Scholar 

  2. Elias JA, Gustilo K, Baeder W, Freundlich B (1987) Synergistic stimulation of fibroblast prostaglandin production by recombinant interleukin 1 and tumor necrosis factor. J Immunol 138:3812–3816

    Google Scholar 

  3. Fransen L, Ruysschaert MR, Van Der Heyden J, Fiers W (1986) Recombinant tumor necrosis factor: Species specificity for a variety of human and murine transformed cell lines. Cell Immunol 100:260–267

    Google Scholar 

  4. Hori K, Ehrke MJ, Mace K, Mihich E (1987) Effect of recombinant tumor necrosis factor on tumoricidal activation of murine macrophages: Synergism between tumor necrosis factor and γ-interferon. Cancer Res 47:5868–5874

    Google Scholar 

  5. Huang JJ, Newton RC, Rutledge SJ, Horuk R, Matthew JB, Covington M, Lin Y (1988) Characterization of murine IL-1β, isolation, expression and purification. J Immunol 140:3838–3843

    Google Scholar 

  6. Jelinek DF, Lipsky PE (1987) Enhancement of human B cell proliferation and differentiation by tumor necrosis factor-α and interleukin 1. J Immunol 139:2970–2976

    Google Scholar 

  7. Kaye J, Janeway CA Jr (1984) Induction of receptors for IL-2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res 3:175–181

    Google Scholar 

  8. Kohase M, Henrikson-DeStefano D, May LT, Vilcek J, Sehgal PB (1986) Induction of beta-interferon by tumor necrosis factor: A homeostatic mechanism in the control of cell proliferation. Cell 45:659–666

    Google Scholar 

  9. Kramer SM, Aggarwal BB, Eessalu TE, McCabe SM, Ferraiolo BL, Figari IS, Palladino MA Jr (1988) Characterization of the in vivo and in vitro species preference of human and murine tumor necrosis factor-α. Cancer Res 48:920–925

    Google Scholar 

  10. Lachman LB, Brown DC, Dinarello CA (1987) Growth-promoting effect of recombinant interleukin 1 and tumor necrosis factor for a human astrocytoma cell line. J Immunol 138:2913–2916

    Google Scholar 

  11. Le J, Vilcek J (1987) Biology of disease. Tumor necrosis factor and interleukin 1: Cytokines with multiple overlapping biological activities. Lab Invest 56:234–248

    Google Scholar 

  12. Mace K, Mayhew E, Mihich E, Ehrke MJ (1988) Alterations in murine host defense functions by Adriamycin or liposome-encapsulated Adriamycin. Cancer Res 48:130–136

    Google Scholar 

  13. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, Gillis S, Henney CS, Kronheim SR, Grabstein K, Conlon PJ, Hopp TP, Cosman D (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315:641–647

    Google Scholar 

  14. Matsushima K, Akahoshi T, Yamada M, Furutani Y, Oppenheim JJ (1986) Properties of a specific interleukin 1 (IL1) receptor on human Epstein-Barr virus-transformed B lymphocytes: Identity of the receptor for IL1-α and IL1-β. J Immunol 136:4496–4502

    Google Scholar 

  15. Mizel SB, Dukovich M, Rothstein J (1983) Preparation of goat antibodies against interleukin 1: Use of an immunoadsorbent to purify interleukin 1. J Immunol 131:1834–1837

    Google Scholar 

  16. Old LJ (1985) Tumor necrosis factor (TNF). Science 230:630–632

    Google Scholar 

  17. Pober JS, Gimbrone MA Jr, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA (1986) Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J Immunol 137:1893–1896

    Google Scholar 

  18. Ranges GE, Figari IS, Espevik T, Palladino MA Jr (1987) Inhibition of cytotoxic T cell development by transforming growth factor β and reversal by recombinant tumor necrosis factor α. J Exp Med 166:991–998

    Google Scholar 

  19. Scheurich P, Thoma B, Ucer U, Pfizenmaier K (1987) Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-α: Induction of TNF receptors on human T cells and TNF-α-mediated enhancement of T cell responses. J Immunol 138:1786–1790

    Google Scholar 

  20. Shirai T, Yamaguchi Y, Ito H, Todd DW, Wallace RB (1985) Cloning and expression in E. coli of the gene for human tumor necrosis factor. Nature 313:803–806

    Google Scholar 

  21. Smith RA, Kirstein M, Fiers W, Baglioni C (1986) Species specificity of human and murine tumor necrosis factor. J Biol Chem 261:14871–14874

    Google Scholar 

  22. Stewart WE II (1984) Heterogeneities of human interferons. In: Mihich E (ed) Biological responses in cancer: progress toward potential applications. Plenum Press, New York, pp 19–44

    Google Scholar 

  23. Sugarman BJ, Aggarwal BB, Hass PE, Figari IS, Palladino MA Jr, Shepard HM (1985) Recombinant human tumor necrosis factor-α: Effects on proliferation of normal and transformed cells in vitro. Science 230:943–945

    Google Scholar 

  24. Takai Y, Wong GG, Clark SC, Burakoff SJ, Herrmann SH (1988) B cell stimulatory factor-2 is involved in the differentiation of cytotoxic T lymphocytes. J Immunol 140:508–512

    Google Scholar 

  25. Yokota S, Geppert TD, Lipsky PE (1988) Enhancement of antigen- and mitogen-induced human T lymphocyte proliferation by tumor necrosis factor-α. J Immunol 140:531–536

    Google Scholar 

  26. Zucali JR, Elfenbein GJ, Barth KC, Dinarello CA (1987) Effects of human interleukin 1 and human tumor necrosis factor on human T lymphocyte colony formation. J Clin Invest 80:772–777

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Grace Cancer Drug Center, Roswell Park Memorial Institute, 666 Elm Street, 14263, Buffalo, NY, USA

    M. Jane Ehrke, Richard L. X. Ho & Kazuyoshi Hori

Authors
  1. M. Jane Ehrke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard L. X. Ho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuyoshi Hori
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported in part by Asahi Chemical Industry Co., Inc. and by USPHS Grants CA-24538, CA-15142 and CA-09072 awarded by the National Cancer Institute, Department of Health and Human Services

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ehrke, M.J., Ho, R.L.X. & Hori, K. Species-specific TNF induction of thymocyte proliferation. Cancer Immunol Immunother 27, 103–108 (1988). https://doi.org/10.1007/BF00200012

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00200012

Keywords

Search

Navigation