Summary
Lymphoblasts from patients with acute lymphatic leukemia were examined for the presence of surface markers and for their capacity to stimulate allogeneic donors in MLC. Lymphoblasts from eight patients, which made E rosettes, consistently failed to stimulate allogeneic donors on at least three separate occasions despite the vigorous response of these same allogeneic donors to remission cells from the patients.
There were eleven patients who had lymphoblasts with no detectable markers or “null” lymphoblasts. Three of these also failed to stimulate in MLC. The “null” lymphoblasts from the remaining eight patients produced vigorous allogeneic responses. Since serologic data is now available suggesting that “null” lymphoblasts from some ALL patients have serologically detectable T cell antigens [16] while others have antigens found predominantly on B cells [20], it is conceivable that the capacity of these cells to stimulate in MLC may distinguish lymphoblasts within the “null” category with those which fail to stimulate representing early T cell precursors and those which do stimulate being early B cell precursors.
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References
Borella, L., Sen, L.: T and B lymphocytes and lymphoblasts in untreated acute lymphocyte leukemia. Cancer 34, 646–654 (1974)
Bowles, C. A., White, G. T., Lucas, B.: Rosette formation by canine peripheral blood lymphocytes. J. Immunol. 114, 399–402 (1975)
Edelson, R. L., Kirkpatrick, C. H., Shevach, E. M., Schein, P. S., Smith, R. W., Green, I., Lutzner, M.: Preferential cutaneous infiltration by neoplastic thymus derived lymphocytes morphologic and functional studies. Ann. Intern. Med. 80, 685–692 (1974)
Fu, S. M., Winchester, R. J., Kunkel, H. G.: The occurrence of the HL-B alloantigens on the cells of unclassified acute lymphoblastic leukemias. J. Exp. Med. 142, 1334–1338 (1975)
Halterman, R. H., Johnson, G. E., Leventhal, B. G.: Storage method for leukemia blast cells. NCI Monograph 37, 149–151 (1973)
Han, T., Minowada, J.: A unique “leukaemic” T lymphoid cell line: absence of stimulating effect in mixed lymphocyte reaction. Clin. Exp. Immunol. 15, 535–541 (1973)
Humphrey, G. B., Lankford, J.: Acute leukemia: The use of surface markers in classification. Sem. Oncol. (in press)
Kersey, J. H., Sabad, A., Peczalska, K., Hallgren, H. M., Juins, E. J., Nesbit, M. E.: Acute lymphoblast leukemia cells with T (thymus derived) lymphocyte markers. Science 182, 1355–1356 (1973)
Komuro, K., Boyse, E. A.: In vitro demonstration of thymic hormone in the mouse by conversion of precursor cells into lymphocytes. Lancet 1, 740–743 (1973)
Lohrmann, H. P., Novikovs, L., Graw, R. G., Jr.: Stimulatory capacity of human T and B lymphocytes in the mixed leukocyte culture. Nature 250, 144–146 (1974)
Lohrmann, H. P., Whang-Peng, J.: Human mixed leukocyte culture: identification of the proliferating lymphocyte subpopulation by sex chromosome markers. J. Exp. Med. 140, 54–60 (1974)
Mann, D. L., Abelson, L., Harris, S., Amos, D. B.: Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera. J. Exp. Med. 142, 84–89 (1975)
Melief, C. J. M., Schweitzer, M., Zeylemaker, W. P., Verhagen, E. H., Eijsvogel, V. P.: Some immunological properties of lymphoid cells from patients with acute lymphatic leukaemia (ALL). Clin. Exp. Immunol. 15, 131–143 (1973)
Mohanakumar, T., Metzgar, R. S.: Human thymus-leukemia related antigen(s): detection by a nonhuman primate antiserum. Cellular Immunol. 12, 30–36 (1974)
Nelson, D. L., Bundy, B. M., Pitchon, H. E., Blaese, R. M., Strober, W.: The effector cells in human peripheral blood mediating mitogen induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity. J. Immunol. (in press)
Niederhuber, J. E., Frelinger, J. A.: Expression of Ia antigens on T and B cells and their relationship to immune response functions. Transplant. Rev. (in press)
Royston, I., Graze, P. R., Pitts, R. B.: Failure of cultured human T-cell lymphoid lines to stimulate in mixed leukocyte culture. J. Nat. Cancer Inst. 53, 361–366 (1974)
Sacks, K. L., Olweny, C., Mann, D. L., Simon, R., Johnson, G. E., Poplack, D. G., Leventhal, B. G.: A clinical trial of chemotherapy and RAJI immunotherapy in advanced acute lymphatic leukemia. Cancer Research 35, 3715–3720 (1975)
Tsukimoto, I., Wong, K. Y., Lampkin, B. C.: Surface markers and prognostic factors in acute lymphoblastic leukemia. New Engl. J. Med. 294, 245–248 (1976)
Weiner, M. S., Bianco, C., Nussenzweig, V.: Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood 42, 939–946 (1973)
Winchester, R. J., Fu, S. M., Wernet, P., Kunkel, H. G., Dupont, B., Jersild, C.: Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J. Exp. Med. 141, 924–929 (1975)
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Leventhal, B.G., Leung, E., Johnson, G. et al. E rosette forming lymphoblasts fail to stimulate allogeneic cells in mixed leukocyte culture (MLC). Cancer Immunol Immunother 2, 21–25 (1977). https://doi.org/10.1007/BF00199087
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DOI: https://doi.org/10.1007/BF00199087