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Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping

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Summary

Two different monoclonal anti-A and anti-B and several different affinity purified Ulex europaeus lectin I reagents were evaluated and compared with conventional anti-A and anti-B sera and Ulex anti-H for serologic properties, in inhibition tests with secretor salivas, and in elution tests with bloodstains. The monoclonal and purified reagents were found to be comparable to conventional ones, and accordingly suitable for forensic inhibition and elution procedures.

Zusammenfassung

Zwei verschiedene monoklonale Anti-A- und Anti-B-Reagenzien sowie verschiedene Lectin-I-Präparate, durch Affinitätschromatographie aus Ulex-europaeus-Samen gereinigt, wurden mit üblichen Anti-A- und Anti-B-Seren sowie Ulex-Anti-H hinsichtlich ihrer serologischen Eigenschaften im Hemmtest mit Sekretor-Speichel und im Elutionstest bei Blutspuren miteinander verglichen. Die monoklonalen Reagenzien und Ulex-Präparate sind mit den bisher üblichen Reagenzien durchaus vergleichbar und eignen sich gleichermaßen für forensische Hemm- und Elutionstests.

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Authors and Affiliations

  1. Forensic Science Laboratories, University of New Haven, 06516, West Haven, CT, USA

    R. E. Gaensslen & J. E. Carroll

  2. Connecticut State Police, Forensic Science Laboratory, 294 Colony Street, 06450, Meriden, CT, USA

    H. C. Lee

Authors
  1. R. E. Gaensslen
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  2. H. C. Lee
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  3. J. E. Carroll
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Additional information

Supported in part by a grant from the University of New Haven Faculty Research Fund

Reported in part at the 9th Annual Meeting of the Northeastern Association of Forensic Scientists, Hasbrouck Heights, NJ, USA, October 1983

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Gaensslen, R.E., Lee, H.C. & Carroll, J.E. Evaluation of monoclonal anti-A and anti-B and affinity-purified Ulex europaeus lectin I for forensic blood grouping. Z Rechtsmed 93, 259–268 (1984). https://doi.org/10.1007/BF00198651

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