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Photosynthetic oxygen evolution and chlorophyll fluorescence in intact isolated chloroplasts on a solid support: the influence of orthophosphate

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Abstract

We devised recently a method to trap intact isolated chloroplasts on a solid support consisting of membrane filters made of cellulose nitrate (Cerović et al., 1987, Plant Physiol. 84, 1249–1251). The addition of alkaline phosphatase to the reaction medium enabled continuous photosynthesis by spinach (Spinacia oleracea L.) chloroplasts to be sustained by hydrolysis of newly produced and exported triose phosphates and recycling of orthophosphate. In this system, simultaneous measurements of chlorophyll fluorescence and oxygen evolution were performed and their dependence on orthophosphate concentration was investigated. Optimal photosynthesis was obtained at a much higher initial orthophosphate concentration (2–4 mM) compared to intact chloroplasts in suspension. Secondary kinetics of chlorophyll fluorescence yield were observed and were shown to depend on the initial orthophosphate concentration.

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Abbreviations

Chl:

chlorophyll

CSS:

intact isolated chloroplasts on solid support

ICS:

intact isolated chloroplasts in suspension

Pi :

orthophosphate

v:

rate of O2 evolution

PPFD:

photosynthetic photon flux density

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Authors and Affiliations

  1. Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11 000, Belgrade, Yugoslavia

    Zoran G. Cerović

  2. Maize Research Institute, Biophysical Laboratory, 89-Zemun, P.O. Box, 11 081, Belgrade, Yugoslavia

    Zeljko Vučinić

  3. Robert Hill Laboratory, University of Sheffield, 26 Taptonville Road, S10 2BR, Sheffield, UK

    David A. Walker

Authors
  1. Zoran G. Cerović
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  2. Zeljko Vučinić
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  3. David A. Walker
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Additional information

The authors wish to thank Dr. Marijana Plesničar, from the University of Novi Sad, for stimulating discussions. This work was supported by the Fond for Science of the Republic of Serbia. Z.G.C.'s visit to the Robert Hill Laboratory was supported by the British Council and the University of Sheffield.

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Cerović, Z.G., Vučinić, Z. & Walker, D.A. Photosynthetic oxygen evolution and chlorophyll fluorescence in intact isolated chloroplasts on a solid support: the influence of orthophosphate. Planta 184, 248–253 (1991). https://doi.org/10.1007/BF00197954

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