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Time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by Fusarium oxysporum f. sp. radicis-lycopersici

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Abstract

The accumulation of hydroxyproline-rich glycoproteins (HRGPs) in cell walls of dicotyledonous plants is thought to be involved in the defense response to pathogens. An antiserum raised against deglycosylated HRGPs from melon was used for studying the subcellular localization of these glycoproteins in susceptible and resistant tomato (Lycopersicon esculentum Mill.) root tissues infected by Fusarium oxysporum f.sp. radicis-lycopersici. A time-course of HRGP accumulation revealed that these glycoproteins increased earlier and to a higher extent in resistant than in susceptible cultivars. In the compatible interaction, increase in HRGPs was largely correlated with pathogen invasion and appeared to occur as a result of wall damage. In the incompatible interaction, HRGPs accumulated in the walls of uninvaded cells, thus indicating a possible role in the protection against fungal penetration. The occurrence of substantial amounts of HRGPs in papillae, known to be physical barriers formed in response to infection, and in intercellular spaces provides additional support to the concept that such glycoproteins play an important role in disease resistance.

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Abbreviations

GAR-gold antibodies:

gold-conjugated goat antiserum to rabbit immunoglobulins

HRP:

deglycosylated hydroxyproline-rich glycoprotein

HRGP:

hydroxyproline-rich glycoprotein

PBS:

phosphate-buffered saline

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Authors and Affiliations

  1. Département de phytologie, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval, G1K 7P4, Sainte-Foy, Québec, Canada

    Nicole Benhamou & Jean Grenier

  2. Centre de Physiologie Végétale, U.A. 241, CNRS, Université Paul-Sabatier, 118, route de Narbonne, F-31062, Toulouse Cédex, France

    Dominique Mazau & Marie-Thérése Esquerré-Tugayé

Authors
  1. Nicole Benhamou
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  2. Dominique Mazau
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  3. Jean Grenier
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  4. Marie-Thérése Esquerré-Tugayé
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Additional information

The present study was supported by grants from the National Sciences and Engineering Research Council of Canada and from the Ministère de l'Éducation du Québec (FCAR). The authors are indebted to S. Noël for excellent technical assistance and to C. Parent for typing this manuscript. Purified tomato HRGP1 (extensin) was kindly provided by Dr D.T.A. Lamport, Michigan State University, East Lansing, USA. Electron-microscope investigations were carried out at the Département de Phytologie, Université Laval, with a JEOL 1200 EX electron microscope.

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Benhamou, N., Mazau, D., Grenier, J. et al. Time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by Fusarium oxysporum f. sp. radicis-lycopersici . Planta 184, 196–208 (1991). https://doi.org/10.1007/BF00197948

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