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Ciliated olfactory receptor neurons in goldfish (Carassius auratus) partially survive nerve axotomy, rapidly regenerate and respond to amino acids

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Abstract

Electro-olfactogram (EOG) recordings in response to amino acid stimulation were made from both control and experimental olfactory mucosae following unilateral axotomy. The recorded EOG amplitudes, amino acid stimulus relative effectiveness and dose-response relations for control and experimental mucosae were comparable in all pre- and postoperative recordings. Semi-thin investigations of olfactory mucosae showed degeneration of olfactory receptors but indicated that intact receptors were also present. SEM of olfactory mucosae revealed that ciliated receptor cells were present in both axotomized and control sides on postoperative days, whereas microvillous receptors completely degenerated and did not regenerate until 7 weeks post axotomy. The present findings along with previous behavioral observations suggest at least three possible sources of the EOGs recorded from the experimental olfactory mucosae following olfactory nerve transection: (1) young olfactory receptor neurons whose axons had not yet reached the region of the transected olfactory nerve; (2) newly-emerged olfactory receptor neurons; and (3) olfactory receptor neurons that had not degenerated.

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Abbreviations

EOG:

electro-olfactogram

SEM:

scanning electron microscopy (micrograph)

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Authors and Affiliations

  1. Physiologisches Institut der Universität, Humboldtallee 23, D-37073, Göttingen, Germany

    H. P. Zippel

  2. Zoologisches Institut der Universität, Luisenstr. 14, D-80333, München, Germany

    T. Lago-Schaaf

  3. Department of Zoology and Physiology, Louisiana State University, 70803, Baton Rouge, LA, USA

    J. Caprio

Authors
  1. H. P. Zippel
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  2. T. Lago-Schaaf
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  3. J. Caprio
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Zippel, H.P., Lago-Schaaf, T. & Caprio, J. Ciliated olfactory receptor neurons in goldfish (Carassius auratus) partially survive nerve axotomy, rapidly regenerate and respond to amino acids. J Comp Physiol A 173, 537–547 (1993). https://doi.org/10.1007/BF00197762

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