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Low-CO2-inducible protein synthesis in the green alga Dunaliella tertiolecta

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Abstract

In the green marine alga Dunaliella tertiolecta, a CO2-concentrating mechanism is induced when the cells are grown under low-CO2 conditions (0.03% CO2). To identify proteins induced under low-CO2 conditions the cells were labelled with 35SO4 2−, and seven polypeptides with molecular weights of 45, 47, 49, 55, 60, 68 and 100 kDa were detected. The induction of these polypeptides was observed when cells grown in high CO2 (5% CO2 in air) were switched to low CO2, but only while the cultures were growing in light. Immunoblot analysis of total cell protein against pea chloroplastic carbonic anhydrase polyclonal antibodies showed immunoreactive 30-kDa bands in both high- and low-CO2-grown cells and an aditional 49-kDa band exclusively in low-CO2-grown cells. The 30-kDa protein was shown to be located in the chloroplast. Western blot analysis of the plasmamembrane fraction against corn plasma-membrane AT-Pase polyclonal antibodies showed 60-kDa bands in both high- and low-CO2 cell types as well as an immunoreactive 100-kDa band occurring only in low-CO2-grown cells. These results suggest that there are two distinct forms of both carbonic anhydrase and plasma-membrane ATPase, and that one form of each of them can be regulated by the CO2 concentration.

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Abbreviations

CA:

carbonic anhydrase

DIC:

dissolved inorganic carbon (CO2+ HCO3 −)

CCM:

CO2-concentrating mechanism

low CO2 :

air containing 0.03% CO2

high CO2 :

air supplemented with 5% CO2 (v/v)

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Authors and Affiliations

  1. Department of Botany, Louisiana State University, 70803, Baton Rouge, LA, USA

    Ziyadin Ramazanov

  2. Instituto de Algologia Aplicada, Universidad de Las Palmas, Box 550, Las Palmas, Gran Canaria, Spain

    Pedro A. Sosa, Miguel Jiménez del Rio, Juan Luis Gómez-Pinchetti & Guillermo García Reina

  3. Department of Microbiology, Louisiana State University, 70803, Baton Rouge, LA, USA

    Margaret C. Henk

Authors
  1. Ziyadin Ramazanov
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  2. Pedro A. Sosa
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  3. Margaret C. Henk
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  4. Miguel Jiménez del Rio
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  5. Juan Luis Gómez-Pinchetti
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  6. Guillermo García Reina
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Additional information

We thank Prof. John Coleman for providing antibodies raised against pea chloroplast CA, Dr. James V. Moroney for providing antibodies raised against the 37-kDa periplasmic carbonic anhydrase of CO2 Chlamydomonas reinhardtii, and Prof. Leonard T. Robert for a gift of corn plasma-membrane 100-kDa ATPase antibodies. We thank Dr. Jeanine Olsen (University of Groningen, the Netherlands) for style comments. This work was supported by the Institute Tecnológico de Canarias (Spain).

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Ramazanov, Z., Sosa, P.A., Henk, M.C. et al. Low-CO2-inducible protein synthesis in the green alga Dunaliella tertiolecta . Planta 195, 519–524 (1995). https://doi.org/10.1007/BF00195709

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  • DOI: https://doi.org/10.1007/BF00195709

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