Abstract
The γ-keto triazole derivative 4,4-dimethyl-1-(2-methoxyphenyl)-1-(1,2,4-triazol-1-yl)-1-penten-3-one is toxic to Nicotiana tabacum L. cv. Xanthi plants or cell cultures. Analysis of the sterol composition of treated wild-type plant material demonstrates that this herbicide is an inhibitor of the C-14α-methyl demethylation process in sterol biosynthesis. Selection experiments, consisting of screening large populations of microcalli derived from UV-mutagenized tobacco protoplasts for resistance to a lethal dose (1 mg · 1−1) of the γ-keto triazole, have resulted in the recovery of two groups of resistant calli. In the first group, selected calli show a sterol composition in the absence or presence of the inhibitor very similar to that of wild-type sensitive calli, whereas in the second group the main feature of the selected calli is a new sterol profile. These calli present an overproduction of sterols with a concomitant esterification of overproduced metaolites, just as it was demonstrated for calli previously selected in our laboratory for resistance to LAB 170250F, a triazole fungicide (Maillot-Vernier et al., 1991, Mol. Gen. Genet. 231, 33–40).
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Abbreviations
- 14DM:
-
cytochrome P-450-dependent obtusifoliol 14α-demethylase
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Schaller, H., Maillot-Vernier, P., Belliard, G. et al. Increased sterol biosynthesis in tobacco calli resistant to a triazole herbicide which inhibits demethylation of 14α-methyl sterols. Planta 187, 315–321 (1992). https://doi.org/10.1007/BF00195654
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DOI: https://doi.org/10.1007/BF00195654