Abstract
We examined the effects of pinealectomy and blinding (bilateral ocular enucleation) on the circadian locomotor activity rhythm in the Japanese newt, Cynops pyrrhogaster. The pinealectomized newts were entrained to a light-dark cycle of 12 h light and 12 h darkness. After transfer to constant darkness they showed residual rhythmicity for at least several days which was gradually disrupted in prolonged constant darkness. Blinded newts were also entrained to a 12 h light/12 h dark cycle. In subsequent constant darkness they showed free-running rhythms of locomotor activity. However, the freerunning periods noticeably increased compared with those observed in the previous period of constant darkness before blinding. In blinded newts entrained to the light/dark cycle the activity rhythms were gradually disrupted after pinealectomy even in the presence of the light/dark cycle. These results suggest that both the pineal and the eyes are involved in the newt's circadian system, and also suggest that the pineal of the newt acts as an extraretinal photoreceptor which mediates the entrainment of the locomotor activity rhythm.
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Abbreviations
- τ :
-
circadian period
- DD:
-
constant darkness
- LD:
-
cycle, light-dark cycle
- LD 12:12:
-
light-dark cycle of 12 h light and 12 h darkness
References
Adler K (1971) Pineal end organ: role in extraoptic entrainment of circadian locomotor rhythm in frogs. In: Menaker M (ed) Biochronometry. Natl Acad Sci, Washington, DC, pp 342–350
Binkley S, Adler K, Taylor DH (1973) Two methods for using period length to study rhythmic phenomena. J Comp Physiol 83:63–71
Binkley SA, Riebman JB, Reilly KB (1978) The pineal gland: a biological clock in vitro. Science 202:1198–1201
Cahill GM, Besharse JC (1990) Circadian regulation of melatonin in the retina of Xenopus laevis: limitation by serotonin availability. J Neurochem 2:716–719
Delgado MJ, Vivien-Roels B (1989) Effect of environmental temperature and photoperiod on the melatonin levels in the pineal, lateral eye, and plasma of the frog, Rana perezi: importance of ocular melatonin. Gen Comp Endocrinol 75:46–53
Demian JJ, Taylor DH (1977) Photoreception and locomotor rhythm entrainment by the pineal body of the newt, Notophthalmus viridescens (Amphibia, Urodela, Salamandridae). J Herpetol 11:131–139
Ebihara S, Kawamura H (1981) The role of the pineal organ and the suprachiasmatic nucleus in the control of circadian locomotor rhythms in Java sparrows, Padda oryzivora. J Comp Physiol 141:207–214
Ebihara S, Uchiyama K, Oshima I (1984) Circadian organization in the pigeon, Columba livia: the role of the pineal organ and the eye. J Comp Physiol A 154:59–69
Falcon J, Marmillon JB, Claustrat B, Collin JP (1989) Regulation of melatonin secretion in a photoreceptive pineal organ: an in vitro study in the pike. J Neurosci 9:1943–1950
Foà A (1991) The role of the pineal and the retinae in the expression of circadian locomotor rhythmicity in the ruin lizard, Podarcis sicula. J Comp Physiol A 169:201–207
Garg SK, Sundararaj BI (1986) Role of pineal in the regulation of some aspects of circadian rhythmicity in the catfish, Heteropneustes fossilis (Bloch). Chronobiologia 13:1–11
Gaston S (1971) The influence of the pineal organ on the circadian activity rhythm in birds. In: Menaker M (ed) Biochronometry. Natl Acad Sci, Washington, DC, pp 541–548
Gaston S, Menaker M (1968) Pineal function: the biological clock in the sparrow? Science 160:1125–1127
Gwinner E (1978) Effects of pinealectomy on circadian locomotor activity rhythms in European starlings, Sturnus vulgaris. J Comp Physiol 126:123–129
Gwinner E, Benzinger I (1978) Synchronization of a circadian rhythm in pinealectomized European starlings by daily injections of melatonin. J Comp Physiol 127:209–213
Iuvone PM, Besharse JC (1983) Regulation of indoleamine N- -acetyltransferase activity in the retina: effects of light and dark, protein synthesis inhibitors and cyclic nucleopeptide analogs. Brain Res 273:111–119
Kavaliers M (1979) Pineal involvement in the control of circadian rhythmicity in the lake chub, Couesius plumbeus. J Exp Zool 209:33–40
Kavaliers M (1981) Circadian organization in white suckers Catostomus commersoni: the role of the pineal organ. Comp Biochem Physiol 68A:127–129
Menaker M (1971) Rhythms, reproduction, and photoreception. Biol Reprod 4:295–308
Menaker M (1985) Eyes — the second (and third) pineal glands? In: Evered D, Clark S (eds) Photoperiodism, melatonin and the pineal. Ciba Foundation symposium 117. Pitman, London, pp 78–92
Menaker M, Wisner S (1983) Temperature-compensated circadian clock in the pineal of Anolis. Proc Natl Acad Sci USA 80:6119–6121
Oksche A, Hartwig HG (1979) Pineal sense organs — components of photoneuroendocrine systems. In: Kappers JA, Pevet P (eds) Progress in brain research vol 52. The pineal gland of vertebrates including man. Elsevier/North-Holland, Amsterdam, pp 113–130
Pittendrigh CS, Daan S (1976) A functional analysis of circadian pacemakers in nocturnal rodents. I. The stability and lability of spontaneous frequency. J Comp Physiol 106:223–252
Tabata M, Minh-Nyo M, Oguri M (1988) Involvement of retinal and extraretinal photoreceptors in the mediation of nocturnal locomotor activity rhythms in the catfish, Silurus asotus. Exp Biol 47:219–225
Takahashi JS, Hamm H, Menaker M (1980) Circadian rhythms of melatonin release from individual superfused chicken pineal glands in vitro. Proc Natl Acad Sci USA 77:2319–2322
Turek FW, McMillan JP, Menaker M (1976) Melatonin: effects on the circadian locomotor rhythm of sparrows. Science 194:1441–1443
Underwood H (1977) Circadian organization in lizards: the role of the pineal organ. Science 195:587–589
Underwood H (1979) Melatonin affects circadian rhythmicity in lizards. J Comp Physiol 130:317–323
Underwood H (1981) Circadian organization in the lizard Sceloporus occidentalis: the effects of pinealectomy, blinding, and melatonin. J Comp Physiol 141:537–547
Underwood H (1983) Circadian organization in the lizard Anolis carolinensis: a multioscillator system. J Comp Physiol 152:265–274
Underwood H, Harless M (1985) Entrainment of the circadian activity rhythm of a lizard to melatonin injections. Physiol Behav 35:267–270
Underwood H, Menaker M (1970) Extraretinal light perception: entrainment of the biological clock controlling lizard locomotor activity. Science 170:190–193
Underwood H, Menaker M (1976) Extraretinal photoreception in lizards. Photochem Photobiol 23:227–243
Underwood H, Siopes T (1984) Circadian organization in Japanese quail. J Exp Zool 232:557–566
Zimmerman NH, Menaker M (1979) The pineal gland: a pacemaker within the circadian system of the house sparrow. Proc Natl Acad Sci USA 76:999–1003
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Chiba, A., Kikuchi, M. & Aoki, K. The effects of pinealectomy and blinding on the circadian locomotor activity rhythm in the Japanese newt, Cynops pyrrhogaster . J Comp Physiol A 172, 683–691 (1993). https://doi.org/10.1007/BF00195394
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DOI: https://doi.org/10.1007/BF00195394