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Jasmonate signalling can be uncoupled from abscisic acid signalling in barley: identification of jasmonate-regulated transcripts which are not induced by abscisic acid

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Abstract

Jasmonate and abscisic acid induce several identical mRNAs and proteins in barley. In order to study whether both hormones act through the same signalling pathway, we identified four transcripts induced by jasmonic acid methylester (JM) in leaf segments of barley (Hordeum vulgare L. cv. Salome). These newly identified transcripts were not induced by abscisic acid within the tested times of 2–72 h. This finding supports the conclusion that jasmonate signalling in barley is independent of abscisic acid, in contrast to the wound-induction signal cascade of proteinase-inhibitor II in tomato and potato. Of the four isolated cDNAs, the putative translation frame of one was homologous to caffeic acid methyltransferase, another was homologous to chalcone synthase, and the C-terminus of the third showed homology to two proteins from rice (a salt-induced protein and a root-specific protein); the last cDNA was not homologous to any sequences in the databases. The new cDNAs will be valuable tools for studying jasmonate signal transduction in barley.

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Abbreviations

ABA:

cis-abscisic acid

CHS:

chalcone synthase

JIP:

jasmonate-induced protein

JM:

jasmonic acid methylester

JRG:

jasmonate-regulated gene

OMT:

O-methyltransferase

ORF:

open reading frame

PCR:

polymerase chain reaction

Pin2:

oteinase inhibitor II

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase

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Authors and Affiliations

  1. Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany

    Justin Lee, Benno Parthier & Marian Löbler

Authors
  1. Justin Lee
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  2. Benno Parthier
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  3. Marian Löbler
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Corresponding author

Correspondence to Marian Löbler.

Additional information

Dedicated to Professor Dr. Dieter Klämbt, Bonn, on the occasion of his 65th birthday.

The cDNA sequences reported in this paper are available in the Genbank under the accession numbers: U43496 (JRG1c18), U43497 (JRG1c34), U43498 (JRG5), JRG10 (U43494), JRG12 (U43495) and JRG14 (U43493).

This work was supported by a grant to M.L. from the Deutsche Forschungsgemeinschaft (DFG).

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Lee, J., Parthier, B. & Löbler, M. Jasmonate signalling can be uncoupled from abscisic acid signalling in barley: identification of jasmonate-regulated transcripts which are not induced by abscisic acid. Planta 199, 625–632 (1996). https://doi.org/10.1007/BF00195196

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  • DOI: https://doi.org/10.1007/BF00195196

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