Skip to main content
SpringerLink
Log in

Immunoresponses to Neisseria meningitidis epitopes: Immunodulation by meningococcus B acts on more than one meningococcal surface epitope

  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

Neisseria meningitidis group B strain M986 (serotypes 2a, 7) (NMB) elicits a specific primary antiphosphorylcholine immune response in mice but not a secondary response. The ability of other serotype and serogroup meningococci to induce similar primary responses in mice was studied, as was the immunogenicity of trinitrophenyl coupled NMB (TNP-NMB) in primary and secondary antitrinitrophenyl responses. Except for NMB, all other strains tested (three serogroup B and one serogroup A meningococcal strains) were found to be very poor phosphorylcholine immunogens. TNP-NMB, however, though proving to be a very good TNP antigen, was only a weak phosphorylcholine antigen. Priming NBF1 female mice with TNP-NMB one month or more before challenging them with the same antigen induced a strong depression of anti-TNP response in the subsequent challenge. However, this effect was not observed with Xid NBF1 male mice. Furthermore, priming mice with NMB weakly affected the anti-TNP response, but greatly depressed the antiphosphorylcholine response, after TNP-NMB challenge. In addition, whereas apparently only one TNP-specific B cell subpopulation was responding in unprimed mice challenged simultaneously with TNP-NMB and TNP-Ficoll (non-additive response), priming mice with NMB appeared to facilitate the independent activation of two different TNP-specific B cell subpopulations (additive response).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Black JR, Black WJ, Cannon JG (1985) Neisserial antigen H. 8 is immunogenic in patients with disseminated gonococcal and meningococcal infections. J Infect Dis 151:650–657

    Google Scholar 

  2. Brodeur BR, Larose Y, Tsang P, Hamel J, Ashton F, Ryan A (1985) Protection against infection with Neisseria meningitidis group B serotype 2b by passive immunization with serotype-specific monoclonal antibody. Infect Immun 50:510–516

    Google Scholar 

  3. Brown AR, Crandall CA (1976) A phosphorylcholine idotype related to TEPC-15 in mice infected with Ascaris suum. J Immunol 116:1105–1109

    Google Scholar 

  4. Cannon JG, Black WB, Nachamkin I, Stewart PW (1984) Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but to most nonpathogenic Neisseria species. Infect Immun 43:994–999

    Google Scholar 

  5. Chesnut RW, Grey HM (1985) Antigen presenting cells and mechanisms of antigen presentation. CRC Crit Rev Immunol 5:263–316

    Google Scholar 

  6. Couderc J, Duquenne C, Sourbier P, Guénet GL, Liacopoulos P (1984) Recovery of in vitro responsiveness of splean cells from CBA/N mice against type 2 thymus-independent (TI-2) antigens. CR Acad Sci Paris 299:281–284

    Google Scholar 

  7. Cunningham AJ, Szenberg A (1968) Further improvements in the plaque technique for detecting single antibody forming cells. Immunology 14:599–606

    Google Scholar 

  8. DeKruyff R, Clayberger C, Fay R, Cantor H (1985) B cell activation: role of dendritic and T cell factors in the response to thymic-independent and -dependent antigens. J Immunol 134:2860–2866

    Google Scholar 

  9. Faro J, Seoane R, Puentes E. Ubeira FM, Regueiro BJ (1985) Immunoresponses to Neisseria meningitidis epitopes: primary versus secondary antiphosphorylcholine responses. Infect Immun 48:428–432

    Google Scholar 

  10. Faro J, Seoane R, Eiras A, Lareo I, Couceiro J, Regueiro BJ (1986) Immunoresponses to Neisseria meningitidis epitopes: suppression of secondary response to phosphorylcholine is carrier specific. Infect Immun 51:224–232

    Google Scholar 

  11. Forsgren S, Kleine B, Pobor G, Martinez-A C, Forni L, Coutinho A (1984) The membrane receptor complex regulating induction and clonal expansion of normal murine B lymphocytes. Rev Infect Dis 6:524–527

    Google Scholar 

  12. Heymer B, Rietschel ETh (1977) Biological properties of peptidoglycans. In: Schlessinger D (ed) Microbiology. American Society for Microbiology, Washington, DC, pp 344–349

    Google Scholar 

  13. Hodes RJ, Hathcock KS, Singer A (1983) Major histocompatibility complex-restricted self-recognition in responses to trinitrophenyl-Ficoll. A novel cell interaction pathway requiring self-recognition of accesory cell H-2 determinants by both T cells and B cells. J Exp Med 157:486–501

    Google Scholar 

  14. Hoffmann MK, Mizell SB, Hirst JA (1984) IL1 requirement for B cell activation revealed by use of adult serum. J Immunol 133:2566–2568

    Google Scholar 

  15. Inman JK (1975) Thymus-independent antigens: the preparation of covalent, hapten-Ficoll conjugates. J Immunol 114:704–709

    Google Scholar 

  16. Jerne NK, Nordin AA (1963) Plaque formation in agar by single antibody-producing cells. Science 140:405

    Google Scholar 

  17. Krause RM (1977) Cell wall antigens of gram-positive bacteria and their biological activities. In: Schlessinger D (ed) Microbiology. American Society for Microbiology, Washington, DC, pp 330–338

    Google Scholar 

  18. Lehningher AL (1982) Principles of biochemistry. Worth, New York, p 322

    Google Scholar 

  19. Letvin NL, Huber BT, Banacerraf B (1982) B-Lymphocyte responses to trinitrophenyl-Ficoll. II. Decreased splenic adherent cell function in mice expressing the xid gene. Cell Immunol 70:241–247

    Google Scholar 

  20. Lüderitz O (1977) Endotoxin and other cell wall components of gram-negative bacteria and their biological activities. In: Schlessinger D (ed) Microbiology. American Society for Microbiology, Washington, DC, pp 239–246

    Google Scholar 

  21. Mackie EB, Longenecker M, Rabin HR, Di Ninno VL, Bryan LE (1982) Immune response of the mouse to Gram-negative bacterial outer membrane extracts as assessed with monoclonal antibodies. J Immunol 129:829–832

    Google Scholar 

  22. McGhee JR, Michalek SM, Babb JL, Kiyo H, Rosenstreich DL, Mergenhaben SE (1980) Lipopolysaccharide regulation of the immune response: cellular basis of adjuvancy and suppression. In: Schlessinger D (ed) Microbiology. American Society for Microbiology, Washington, DC, pp 49–54

    Google Scholar 

  23. Moreno C, Hewitt J, Hastings K, Brown D (1983) Immunological properties of monoclonal antibodies specific for meningococcal polysaccharides: the protective capacities of IgM antibodies specific for polysaccharide group B. J Gen Microbiol 129:2451–2456

    Google Scholar 

  24. Morse SA, Cacciapouti AF, Lysko PG (1979) Physiology of Neisseria gonorrhoeae. Adv Microb Physiol 20:251–320

    Google Scholar 

  25. Nariuchi H, Kakiuchi T (1982) Antibody responses to various T-independent antigens of B-cells with or without C3 receptors and adherent cell requirement for these responses. J Immunol 128:161–167

    Google Scholar 

  26. Phipps RP, Scott DW (1983) A novel role for macrophages: the ability of macrophages to tolerize B cells. J Immunol 131:2122–2127

    Google Scholar 

  27. Schwab JH (1977) Modulation of the immune response by bacteria. In: Schlessinger D (ed) Microbiology. American Society for Microbiology, Washington, DC, pp 366–373

    Google Scholar 

  28. Schwab JH (1979) Acute and chronic inflamation induced by bacterial cell wall structures. In: Schlessinger D (ed) Microbiology. American Society for Microbiology, Washington, DC, pp 209–214

    Google Scholar 

  29. Senff LM, Wegener WS, Brooks GF, Finnerty WR, Makula RA (1976) Phospholipid composition and phospholipase A activity of Neisseria gonorrhoeae. J Bacteriol 127:874–880

    Google Scholar 

  30. Sud IJ, Feingold DS (1975) Phospholipids and fatty acids of Neisseria gonorrhoeae. J Bacteriol 124:713–717

    Google Scholar 

  31. Sugasawara RJ, Prato C, Sippel JE (1983) Monoclonal antibodies against Neisseria meningitidis lipopolysaccharides. Infect Immun 42:863–868

    Google Scholar 

  32. Unanue ER (1981) The regulatory role of macrophages in antigenic stimulation. Part two: symbiotic relationship between lymphocytes and macrophage. Adv Immunol 31:1–136

    Google Scholar 

  33. Williams KR, Claflin JL (1980) Clonotypes of antiphosphocholine antibodies induced with Proteus morganii (Potter). I. Structural and idiotypic similarities in a diverse repertoire. J Immunol 125:2429–2436

    Google Scholar 

  34. Yoshida K, Ohtomo T (1984) Alteration of capsular type of encapsulated strains of Staphylococcus aureus during freeze-drying and storage. Experientia 40:93–94

    Google Scholar 

  35. Zollinger WD, Mandrell RD (1983) Importance of complement source in bactericidal activity of human antibody and murine monoclonal antibody to meningococcal group B polysaccharide. Infect Immun 40:257–264

    Google Scholar 

  36. Zollinger WD, Moran EE, Connelly H, Mandrell RE, Brandt B (1984) Monoclonal antibodies to serotype 2 and serotype 15 outer membrane proteins of Neisseria meningitidis and their use in serotyping. Infect Immun 46:260–266

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Microbiología, Facultad de Medicina, Universidad de Santiago, Santiago de Compostela, Galicia, Spain

    J. Faro, R. Seoane, I. Lareo, A. Eiras, J. Couceiro & B. J. Regueiro

Authors
  1. J. Faro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Seoane
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Lareo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Eiras
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Couceiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. J. Regueiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Faro, J., Seoane, R., Lareo, I. et al. Immunoresponses to Neisseria meningitidis epitopes: Immunodulation by meningococcus B acts on more than one meningococcal surface epitope. Med Microbiol Immunol 176, 131–141 (1987). https://doi.org/10.1007/BF00193894

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00193894

Keywords

Search

Navigation