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Effects of abscisic acid analogues on abscisic acid-induced gene expression in barley aleurone protoplasts: Relationship between structure and function of the abscisic acid molecule

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Abstract

The plant hormone abscisic acid (ABA) mediates gene expression in barley aleurone protoplasts. In order to elucidate the essential functional groups of the ABA molecule, the specificity of a number of ABA analogues for inducing ABA-regulated gene (e.g., RAB, BASI) expression in barley aleurone protoplasts was studied. These analogues have modifications at three different positions of the ABA molecule: (a) the 1′-hydroxyl group (1′-deoxy ABA), (b) the carboxyl group (ABA-methyl ester or ABA-glucose ester), and (c) both the 1′-hydroxyl and 4′-carbonyl groups (α-ionylidene acetic acid). The importance of the different putative functional groups was analyzed. The dose-response analysis of ABA analogues upon the induction gene expression showed the following order: ABA > ABA methyl ester > 1′-deoxy ABA > ABA glucose ester > α-ionylidene acetic acid > α-β-ionone.

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Authors and Affiliations

  1. Department of Molecular Plant Biotechnology, Centre for Phytotechnology RUL-TNO, Leiden, The Netherlands

    R. M. Van der Meulen, F. Heidekamp & M. Wang

  2. Department of Chemistry, Bioorganic Chemistry Unit, University of Bremen, Bremen, Germany

    B. Jastorff

  3. Department of Biological Sciences, The University College of Wales, Aberystwyth Dyfed, UK

    R. Horgan

Authors
  1. R. M. Van der Meulen
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  2. F. Heidekamp
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  3. B. Jastorff
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  4. R. Horgan
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  5. M. Wang
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Van der Meulen, R.M., Heidekamp, F., Jastorff, B. et al. Effects of abscisic acid analogues on abscisic acid-induced gene expression in barley aleurone protoplasts: Relationship between structure and function of the abscisic acid molecule. J Plant Growth Regul 12, 13–19 (1993). https://doi.org/10.1007/BF00193673

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  • DOI: https://doi.org/10.1007/BF00193673

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