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The pineal complex and melatonin affect the expression of the daily rhythm of behavioral thermoregulation in the green iguana

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Abstract

Daily variation in the body temperature of the green iguana (Iguana iguana) was studied by telemetry in laboratory photo-thermal enclosures under a 12Light∶12Dark (L∶D) photoperiod. The lizards showed robust daily rhythms of thermoregulation maintaining their body temperatures (Tb) at higher levels during the day than during the night. Some animals maintained rhythmicity when kept in constant darkness. On light∶dark cycles parietalectomy produced only a transient increase of median Tb in the first or second night following the operation. Pinealectomized lizards on the other hand maintained their body temperatures at significantly lower levels during the day and at significantly higher levels during the night than did sham-operated or intact lizards. This effect was apparently permanent, since one month after pinealectomy lizards still displayed the altered pattern. Plasma melatonin levels in intact animals were high during the night and low during the day and were unaffected by parietalectomy. Pinealectomized lizards showed low levels of plasma melatonin during both the day and the night. A daily intraperitoneal injection of melatonin in pinealectomized animals given a few minutes after the light to dark transition decreased the body temperatures selected by the lizards during the night and increased the body temperatures selected during the following day. Control injections of saline solution had no effect. The significance of these results is discussed in relation to the role of the pineal complex and melatonin in the mediation of thermoregulatory behavior.

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Abbreviations

L∶D :

Light∶Dark

T b :

body temperature

PAR-X :

parietalectomy

PIN-X :

pinealectomy

References

  • Avery RA (1982) Field studies of body temperatures and thermoregulation. In: Gans C, Pough FH (eds) Biology of the Reptilia 12, Physiology C, Physiological Ecology. Academic Press, London, pp 93–166

    Google Scholar 

  • Barber BJ, Crawford EC (1977) A stochastic dual-limit hypothesis for behavioral thermoregulation in lizards. Physiol Zool 50: 53–60

    Google Scholar 

  • Berk ML, Heath JE (1975a) An analysis of behavioural thermoregulation in the lizard, Dipsosaurus dorsalis. J Therm Biol 1: 15–22

    Google Scholar 

  • Berk ML, Heath JE (1975b) Effects of preoptic, hypothalamic and telencephalic lesions on thermoregulation in the lizard, Dipsosaurus dorsalis. J Therm Biol 1: 56–78

    Google Scholar 

  • Cothran ML, Hutchison VH (1979) Effect of melatonin on thermal selection by Crotaphytus collaris (Squamata: Iguanidae). Comp Biochem Physiol 63A: 461–466

    Google Scholar 

  • Cowgell J, Underwood H (1979) Behavioral thermoregulation in lizards: a circadian rhythm. J Exp Zool 210: 189–194

    Google Scholar 

  • Dodt E (1973) The parietal eye (pineal and parietal organs) of lower vertebrates. In: Jung (ed) Handbook of sensory physiology. VII/3B. Springer, Berlin Heidelberg New York, pp 113–140

    Google Scholar 

  • Engbretson GA (1992) Neurobiology of the lacertilian parietal eye system. Ethol Ecol Evol 4: 89–107

    Google Scholar 

  • Engbretson GA, Hutchison VH (1976) Parietal eye-pineal interactions in the lizard Sceloporus occidentalis. J Herpertol 12: 83–87

    Google Scholar 

  • Engbretson GA, Reiner A, Brecha N (1981) Habenular asymmetry and central connection of the parietal eye of lizard. J Comp Neurol 198: 155–165

    Google Scholar 

  • Firth BT, Kennaway DI (1980) Plasma melatonin levels in the scincid lizard Trachydosaurus rugosus. The effects of parietal eye and lateral eye impairment. J Exp Biol 85: 311–321

    Google Scholar 

  • Firth BT, Kennaway DJ (1987) Melatonin content of pineal, parietal eye and blood plasma of the lizard, Trachydosaurus rugosus: effect of constant and fluctuating temperature. Brain Res 404: 313–318

    Google Scholar 

  • Firth BT, Kennaway DJ (1989) Thermoperiod and photoperiod interact to affect the phase of plasma melatonin rhythm in the lizard, Tiliqua rugosa. Neurosci Lett 106: 125–130

    Google Scholar 

  • Firth BT, Turner JS (1982) Sensory, neural and hormonal aspects of thermoreguation. In: Gans C, Pough FH (eds) Biology of the Reptilia 12, Physiology C, Physiological Ecology. Academic Press, Longon, pp 213–274

    Google Scholar 

  • Firth BT, Mauldin RE, Ralph CL (1988) The role of the pineal complex in behavioural thermoregulation in the collared lizard Crotaphytus collaris under seminatural conditions. Physiol Zool 61: 176–185

    Google Scholar 

  • Firth BT, Turner JS, Ralph CL (1989) Thermoregulatory behaviour in two species of iguanid lizards (Crotaphytus collaris and Sauromalus obesus): diel variation and the effect of pinealectomy. J Comp Physiol B 159: 13–20

    Google Scholar 

  • Hammel HT, Caldwell FT, Abrams FM (1967) Regulation of body temperature in the blue-tongue lizard. Science 156: 1260–1263

    Google Scholar 

  • Hutchison VH, Kosh RJ (1974) Thermoregulatory function of the parietal eye in the lizard Anolis carolinensis. Oecologia 16: 173–177

    Google Scholar 

  • Innocenti A, Minutini L, Foà A (1993) The pineal and circadian rhythms of temperature selection and locomotion in lizards. Physiol Behav 53: 911–915

    Google Scholar 

  • Kappers JA (1967) The sensory innevartion of the pineal organ in the lizard, Lacerta viridis, with remarks on its position in the trend of pineal phylogenetic structural and functional evolution. Z Zellforsch 81: 581–618

    Google Scholar 

  • Kluger MH, Tarr RS, Heath JE (1973) Posterior hypothalamic lesions and disturbances in behavioural thermoregulation. Physiol Zool 46: 79–86

    Google Scholar 

  • Korf HW, Wagner U (1981) Nervous connections of the parietal eye in adult Lacerta s. sicula Refinesque as demonstrated by anterograde and retrograde transport of horseradish peroxidase. Cell Tissue Res 219: 567–583

    Google Scholar 

  • Menaker M, Wisner S (1983) Temperature compensated circadian clock in the pineal of Anolis carolinensis. Proc Natl Acad Sci USA 80: 6119–6122

    Google Scholar 

  • Myhre RD, Hammel HT (1969) Behavioural regulation of internal temperature in the lizard, Tiliqua scincoides. Am J Physiol 217: 1490–1495

    Google Scholar 

  • Phillips JA, Harlow HJ (1981) Elevation of upper voluntary temperatures after shielding the parietal eye of the horned lizards. Herpetologica 37: 199–205

    Google Scholar 

  • Phillips JA, Harlow HJ, Ralph CL (1980) Set-point shifts of behavioral thermoregulation in horned lizards after parietal eye manipulation. Am Zool 20: 732

    Google Scholar 

  • Ralph CL, Firth BT, Turner JS (1979) The role of the pineal body in ectotherm thermoregulation. Am Zool 19: 273–293

    Google Scholar 

  • Regal PJ (1967) Voluntary hypothermia in reptiles. Science 155: 1551–1153

    Google Scholar 

  • Rismiller PD (1987) Thermal biology of Lacerta viridis: seasonal aspects. PhD thesis, Philipps-Universität, Marburg/Lahn, Germany

    Google Scholar 

  • Rismiller PD, Heldmaier G (1987) Melatonin and photoperiod affect body temperature selection in the lizard Lacerta viridis. J Therm Biol 12: 131–134

    Google Scholar 

  • Rollag M, Niswender GD (1976) Radioimmunoassay of serum concentration of melatonin in sheep exposed to different lighting conditions. Endocrinology 98: 482–489

    Google Scholar 

  • Saarela S, Reiter RS (1994) Function of melatonin in thermoregulatory processes. Life Sciences 54: 295–311

    Google Scholar 

  • Siegel S, Castellan NJ (1988) Nonparametric statistics for behavioral sciences. McGraw-Hill, New York, USA

    Google Scholar 

  • Sievert LM, Hutchison VH (1989) The parietal eye and thermoregulatory behavior of Crotaphytus collaris (Squamata: Iguanidae). Comp Biochem Physiol 94A: 339–343

    Google Scholar 

  • Skinner DC (1991) Effects of intraperitoneal melatonin injections on the thermoregulation in the transvaal girdled lizard, Cordylus vittifer. J Ther Biol 16: 179–184

    Google Scholar 

  • Sokolove PG, Bushell WN (1978) The chi square periodogram: Its utility for analysis of circadian rhythmicity. J Theor Biol 72: 131–160

    Google Scholar 

  • Stebbins RC (1960) Effects of pinealectomy on the western fence lizard Sceloporus occidentalis. Copeia 1960: 276–283

    Google Scholar 

  • Tosini G, Avery RA (1994a) Diel variation in thermoregulatory set points of the lizard Podarcis muralis. Amphibia-Reptilia 15: 93–96

    Google Scholar 

  • Tosini G, Avery RA (1994b) Occlusion of the parietal eye induces a transient wavelength-dependent shift in a lizard thermoregulatory set points. J Exp Zool 269: 84–87

    Google Scholar 

  • Tosini G, Foá A, Avery RA (1996) Pinealectomy increases thermoregulatory set point in the lacertid lizard Podarcis sicula. J Herpertol 30: 104–106

    Google Scholar 

  • Underwood H (1985) Pineal melatonin rhythms in the lizard Anolis carolinensis: effects of light and temperature cycles. J Comp Physiol A 157: 57–65

    Google Scholar 

  • Underwood H (1992) Endogenous rhythms. in: Gans C, Crew D (eds) Biology of the Reptilia 18, Hormones, Brain, and Behavior. The University of Chicago Press, Chicago London, pp 229–297

    Google Scholar 

  • Wiechmann AF, Wirsig-Weichmann CR (1994) Melatonin receptors distribution in the brain and retina of a lizard, Anolis carolinensis. Brain Behav Evol 43: 26–33

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology and NSF Center for Biological Timing, University of Virginia, Gilmer Hall, 22903, Charlottesville, VA, USA

    G. Tosini & M. Menaker

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  1. G. Tosini
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  2. M. Menaker
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Tosini, G., Menaker, M. The pineal complex and melatonin affect the expression of the daily rhythm of behavioral thermoregulation in the green iguana. J Comp Physiol A 179, 135–142 (1996). https://doi.org/10.1007/BF00193441

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