Abstract
We examined the effects of brassinosteroids on Arabidopsis thaliana (L.) Henyh. ecotype Columbia in order to develop a model system for studying gene regulation by plant steroids. Submicromolar concentrations of two brassinosteroids, brassinolide and 24-epibrassinolide, stimulated elongation of Arabidopsis peduncles and inhibited root elongation, respectively. Furthermore, brassinolide altered the abundance of specific in vitro translatable mRNAs from peduncles and whole plants of Arabidopsis. Root elongation in the auxin-insensitive Arabidopsis mutant axr1 was inhibited by 24-epibrassinolide but not by 2,4-D, indicating an independent mode of action for these growth regulators in this physiological response.
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Abbreviations
- BR:
-
brassinolide
- EBR:
-
24-epibrassinolide; 2.4-D,2,4-dichlorophenoxyacetic acid
- KPSC:
-
10 mM potassium phosphate, pH 6.0, 2% sucrose, 50 μg/ml chloramphenicol
- PAGE:
-
polyacrylamide gel electrophoresis
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Clouse, S.D., Hall, A.F., Langford, M. et al. Physiological and molecular effects of brassinosteroids on Arabidopsis thaliana . J Plant Growth Regul 12, 61 (1993). https://doi.org/10.1007/BF00193234
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DOI: https://doi.org/10.1007/BF00193234