Summary
Our investigations on Canna indica L. indicate that the pollen of this species is polymorphic: there are two types of pollen — a larger type and a comparatively smaller type. Transmission electron microscopy (TEM) revealed the presence of small vacuoles containing tannic substances in the generative cell (GC) of the larger grains: the GC of the mature grain contained a higher quantity of tannins than the GC of the immature grain. Mitochondria, lipid bodies, rough endoplasmic reticulum (RER) and microtubular bundles were present in the cytoplasm of the GC. Numerous mitochondria, lipid bodies and plastids were also present in the vegetative cell (VC), with the mitochondria clustered around the vegetative nucleus. The plastids were observed to be associated with the RER cisterns. During the maturation process, the number of starch grains contained in the plastids decreased.
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References
Dahlgren RMT, Clifford HT, Yeo PF (1985) The families of the monocotyledons: structure, evolution and taxonomy. Springer, Berlin Heidelberg New York
Heslop-Harrison Y (1977) The pollen-stigma interaction: pollen tube penetration in Crocus. Ann Bot (London) 41:913–922
Heslop-Harrison J (1987) Pollen germination and pollen-tube growth. Int Rev Cytol 107:1–78
Heslop-Harrison J, Heslop-Harrison Y (1985) The secretory system of the stigma of Oenothera organensis Munz: some developmental and quantitative features. Isr J Bot 34:187–204
Heslop-Harrison J, Heslop-Harrison Y, Shivanna KR (1984) The evaluation of pollen quality, and a further appraisal of the fluoromatic (FCR) test procedure. Theor Appl Genet 67:367–375
Kress WJ, Stone DE (1982) Nature of the sporoderm in monocotyledons, with special reference to the pollen grains of Canna and Heliconia. Grana 21:129–148
Ledbetter MC, Porter KR (1970) Introduction to the fine structure of plant cells. Springer, Berlin Heidelberg New York
Mathew PM, Philip O (1983) Advances in pollen spore research, vol 10. Today and Tomorrow's Printers & Publishers, New Delhi
Nair PKK (1960) Pollen grains of cultivated plants, I. Canna. J Indian Bot Soc 39:373–381
O'Brien JP, McCully ME (1981) The study of plant structure. Principles and selected methods. Termacharphi, Melbourne
Rowley JR, Skvarla JJ (1975) The glycocalyx initiation of exine spinules on microspores of Canna. Am J Bot 62:479–485
Rowley JR, Skvarla JJ (1986) Development of the pollen grain wall in Canna. Nord J Bot 6:39–65
Russell A, Kaustinen P, Kaustinen HM (1976) Differential staining of tannin in sections of epoxy-embedded plant cells. Stain Technol 51:237–240
Skvarla JJ, Kelly AG (1971) Dictyosome development during microsporogenesis in Canna generalis. In: Heslop-Harrison J (ed) Pollen development and physiology. Butterworths, London, pp 62–67
Skvarla JJ, Rowley JR (1970) The pollen wall of Canna and its similarity to the germinal apertures of other pollen. Am J Bot 57:519–529
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Van Went J, Gori P (1989) The ultrastructure of Capparis spinosa pollen grains. J Submicrosc Cytol Pathol 21:149–156
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Chen, F., Ciampolini, F., Tiezzi, A. et al. The ultrastructure of polymorphic pollen grains of Canna indica L.. Sexual Plant Reprod 2, 193–198 (1989). https://doi.org/10.1007/BF00192766
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DOI: https://doi.org/10.1007/BF00192766