Abstract
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1.
A previous study implies that diacylglycerols (DAGs) released by the phototransduction cascade in photoreceptors of a crab, Leptograpsus, activate protein kinase Cs (PKCs). PKCs were suggested to phosphorylate nuclear proteins that regulate the transcription of rhabdomeral membrane precursors.
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2.
We show that a transcription inhibitor, actinomycin D, reduces the rhabdomeral renewal induced by a diacylglycerol lipase inhibitor, U-57908, but does not eliminate it.
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3.
Within the particular experimental protocol, rhabdomeral maintenance and renewal by controls are inhibited by actinomycin D in an absence of U-57908.
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4.
We conclude that although rhabdomeral renewal induced by PKC activation involves the regulation of transcription, multiple pathways are implicated.
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5.
An alternative route for the degradation of DAGs by phosphorylation is eliminated because a specific inhibitor of DAG kinases, R-59022, exerts no effect.
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6.
U-57908 promotes photoreceptor shrinkage which is not compromised by actinomycin D. Shrinkage occurs independently of rhabdomeral renewal, suggesting a further consequence of PKC activation.
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Abbreviations
- DAG:
-
diacylglycerol
- OKA:
-
okadaic acid
- PKC:
-
protein kinase Cs
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Blest, A.D., Stowe, S. & Delaney, A. A transcription inhibitor, actinomycin D, partially inhibits the rhabdomeral renewal induced by a diacylglycerol lipase inhibitor, U-57908 in crab retinas. J Comp Physiol A 174, 747–754 (1994). https://doi.org/10.1007/BF00192724
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DOI: https://doi.org/10.1007/BF00192724