Summary
We have investigated the effect of systemic treatment with drugs that affect the cAMP cascade on the sensory response and sensory fatigue in an identified mechanosensory neuron of Drosophila. Forskolin, an activator of adenylate cyclase, decreases the sensory response of the neuron. H7, an inhibitor of protein kinase, inhibits sensory fatigue. Octopaminergic ligands facilitate sensory fatigue. These results, together with our previous neurogenetic analysis of sensory fatigue in Drosophila (Corfas and Dudai 1990), corroborate the hypothesis that the cAMP cascade is involved in the generation and modulation of sensory fatigue.
Similar content being viewed by others
Abbreviations
- ANP :
-
antero-notopleural (neuron)
- CDMF :
-
chlordimeform
- ISI :
-
interstimulus interval
References
Baumann KI, Hamann W, Yung WH (1987) Lithium and the excitability of slowly adapting type I (s.a. I) cutaneous mechanoreceptors of the rat. J Physiol 390:38P
Byers D, Davis RL, Kiger JA (1981) Defect in cyclic AMP phosphodiesterase due to the dunce mutations of learning in Drosophila melanogaster. Nature 289:79–81
Corfas G, Dudai Y (1990) Adaptation and fatigue of a mechanosensory neuron in wild-type Drosophila and in memory mutants. J Neurosci 10:491–499
Daly JW (1984) Forskolin, adenylate cyclase, and cell physiology: an overview. Adv Cycl Nucleot Prot Phosphoryl Res 17:81–89
Dudai Y (1987) The cAMP cascade in the nervous system: Molecular sites of action and possible relevance to neuronal plasticity. CRC Crit Rev Biochem 22:221–281
Dudai Y, Sher B, Segal D, Yovell Y (1985) Defective responsiveness of adenylate cyclase to forskolin in the Drosophila memory mutant rutabaga. J Neurogenet 2:365–380
Dudai Y, Buxbaum J, Corfas G, Ofarim M (1987) Formamidines interact with Drosophila octopamine receptors, alter the flies' behavior and reduce learning ability. J Comp Physiol A 161:739–746
Hagiwara S, Kusano K, Saito S (1960) Membrane changes in crayfish stretch receptor neurones during synaptic inhibition and under action of gamma-amino-butyric acid. J Neurophysiol 23:505–515
Hidaka H, Inagaki M, Kawamoto S, Yasuharu S (1984) Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochem 23:5036–5041
Leake LD, Walker RJ (1980) Invertebrate neuropharmacology. Blackie, Glasgow
Lewis EG (1960) A new standard food medium. Drosophila Inf Ser 34:117–118
Malinow R, Madison DV, Tsien RW (1988) Persistent protein kinase underlying long-term potentiation. Nature 335:820–824
Nestler EJ, Greengard P (1984) Protein phosphorylation in the nervous system, Wiley, New York
Pasztor VM, Bush BMH (1983) Graded potentials and spiking in single units of the oval organ, a mechanoreceptor in the lobster ventilatory system. III. Sensory habituation to repetitive stimulation. J Exp Biol 107:465–472
Pasztor VM, Bush BMH (1989) Primary afferent responses of a crustacean mechanoreceptor are modulated by proctolin, octopamine and serotonin. J Neurobiol 20:234–254
Roberts A, Hayes BP (1977) The anatomy and function of free nerve endings in an amphibian skin sensory system. Proc R Soc Lond B 196:415–429
Toshinori H, Garber SS, Aldrich RW (1988) Effect of forskolin on voltage-gated K+ channels is independent of adenylate cyclase activation. Science 240:1652–1655
Tully T, Quinn WG (1985) Classical conditioning and retention in normal and mutant Drosophila melanogaster. J Comp Physiol A 157:263–277
Wagoner PK, Pallota BS (1988) Modulation of acetylcholine receptor desensitization by forskolin is independent of cAMP. Science 240:1655–1658
Watts RE, French AS (1985) Sensory transduction in dorsal cutaneous mechanoreceptors of the frog, Rana pipiens. J Comp Physiol A 157:657–665
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Corfas, G., Dudai, Y. Pharmacological evidence for the involvement of the cAMP cascade in sensory fatigue in Drosophila . J Comp Physiol A 167, 437–440 (1990). https://doi.org/10.1007/BF00192579
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00192579