Abstract
Treating kraft pulps with the crude xylanase from Streptomyces roseiscleroticus followed by alkali extraction reduces the kappa number in a linear manner with enzyme doses up to about 3 IU/gm of oven-dry pulp. The enzyme complex consists of four isoenzymes designated Xyl1, Xyl2, Xyl3 and Xyl4. Each can release chromophores when used alone and each can facilitate alkali extraction to reduce the kappa number, but their relative abilities are different. Of the four isozymes, Xyl4 releases the least color and 237-nm-absorbing material whereas Xyl3 releases the most. Xyl4 best enhances the ability of alkali to reduce the kappa number. The UV absorption spectrum of the material released by alkali extraction differs significantly from the spectral characteristics of that released during enzyme treatment. The alkali-solubilized material has a maximum absorptivity at 265 nm and relatively little absorptivity at 237 nm. The material released during enzyme treatment absorbs strongly at 205 and 237 nm. UV/VIS spectroscopy of the enzyme- or alkali-released material does not show a characteristics lignin peak at 280 nm, nor does it reveal any notable peaks in the visible region. Analysis of the material released by enzyme treatment revealed more than 40 product peaks after fractionation by reversed-phase HPLC. We observed many products with strong UV absorption. These were relatively hydrophilic. Fewer products absorbed in the visible region. These were more hydrophobic. All four isoenzymes exhibit endo-action patterns; none forms xylose from oat-spelt xylan. The action patterns fell into two groups: endo-1 enzymes (Xyl1 and Xyl3) formed xylotriose (X3) and other lower oligosaccharides as the predominant products; endo-2 enzymes (Xyl2 and Xyl4) formed roughly equimolar amounts of X3, xylotetraose (X4), and xylopentaose (X5), and tended to leave larger amounts of undigested higher oligosaccharides.
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References
Anon (1991) Method no. UM 246, TAPPI useful methods. TAPPI, Atlanta, pp 43–44
Bastawde KB, Tabatabai LB, Meagher MM, Srinivasan MC, Vartak HG, Rele MV, Reilly PJ (1991) Catalytic properties and partial amino acid sequence of an actinomycete endo-(1→4)-β-d-xylanase from chainia species. ACS Symp Ser 460:152–168
Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding. Anal Biochem 72:248–254
Clark TA, McDonald AG, Senior DJ, Mayers PR (1990) Mannanase and xylanase treatment of softwood chemical pulps: effects on pulp properties and bleachability. In: Kirk TK, Chang H-m (eds) Biotechnology in pulp and paper manufacture. Butterworth-Heinemann, Boston, pp 153–167
Gierer J, Wännström S (1984) Formation of alkali-stable C-C bonds between lignin and carbohydrate fragments during kraft pulping. Holzforschung 38:181–184
Grabski AC, Jeffreis TW (1991) Production, purification and characterization of β-(1–4)-endoxylanase of Streptomyces roseiscleroticus. Appl Environ Microbiol 57:987–992
Grabski AC, Patel R, Forrester IT, Jeffries TW (1993) Characterization and N-terminal amino acid sequences of β-(1–4)-endoxylanases from Streptomyce roseiscleroticus: purification incorporating a bioprocessing agent. Protein Express Purif 4:120–129
Jiang J-e, Chang H-m (1987) Characterization of residual lignins isolated from unbleached and semibleached softwood kraft pulp. J Wood Chem Technol 7:81–96
Kantelinen A, Rättö M, Sundquist J, Ranua M, Viikari L, Linko M (1988) Hemicellulases and their potential role in bleaching. In: 1988 TAPPI International Pulp Bleaching Conference, Orlando, pp 1–9
Kantelinen A, Sundquist J, Lindo M, Viikari L (1991) The role of reprecipitated xylan in the enzymatic bleaching of kraft pulp. In: Proceedings of the 6th International Symposium of Wood Paper Chemistry, Melbourne, pp 493–500
Kluepfel D, Vats-metha S, Aumont F, Shareck F, Morosoli R (1990) Purification and characterization of a new xylanase (xylanase B) produced by Streptomyces lividans 66. Biochem J 267:45–50
Kluepfel D, Daigneault N, Morosoli R, Shareck F (1992) Purification and characterization of a new xylanase (xylanase C) produced by Streptomyces lividans 66 Appl Microbiol Biotechnol 36:626–631
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lumba FL, Penninckx MJ (1992) Characterization of the multiple forms of β-xylanase produced by a Streptomyces sp. growing on lignocellulose. Appl Microbiol Biotechnol 36:733–738
Manoir JR du, Hamilton J, Senior DJ, Bernier RL, Grant JE, Moser LE (1991) Biobleaching of kraft pulps with cellulase-free xylanase. In: Proceedings of the International Pulp Bleaching Conference, Stockholm. pp 123–138
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Morosoli R, Bertrand J-L, Monou F, Shareck F, Kluepfel D (1986) Purification and properties of a xylanase from Streptomyces lividans. Biochem J 239:587–592
Paice MG, Bernier R Jr, Jurasek L (1988) Viscosity-enhancing bleaching of hardwood kraft pulp with xylanase from a cloned gene. Biotechnol Bioeng 32:235–239
Paice MG, Gurnagul N, Page DH, Jurasek L (1992) Mechanism of hemicellulose-directed prebleaching of kraft pulps. Enzyme Microb Technol 14:272–276
Shareck F, Roy C, Yaguchi M, Morosoli R, Kluepfel D (1991) Sequences of three genes specifying xylanases in Streptomyces lividans. Gene 107:75–82
Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Viikari L, Ranua M, Kantelinen A, Sundquist J, Linko M (1986) Bleaching with enzymes. Proceedings of the 3rd International Conference on Biotechnology in the Pulp and Paper Industry, Stockholm, June 16–19, pp 67–69
Viikari L, Ranua M, Kantelinen A, Linko M, Sundquist J (1987) Application of enzymes in bleaching. In: Proceedings of the 4th International Symposium on Wood Pulping Chemistry, Paris, April 27–30, vol 1, pp 151–154
Viikari L, Kantelinen A, Poutanen K, Ranua M (1990) Characterization of pulps treated with hemicellulolytic enzymes prior to bleaching. In: Kirk TK, Chang H-m (eds) Biotechnology in pulp and paper manufacture. Butterworth-Heinemann, Boston, pp 145–151
Yamasaki T, Hosoya S, Chen C-L, Gratzl JS Chang H-m (1981) Characterization of residual lignin in kraft pulp. In: The Ekman Days International Symposium on Wood and Pulping Chemistry, Stockholm, June 9–12, vol 2, pp 34–42
Zimmerman W (1991) Hemicellulolytic enzyme systems from actinomycetes. In: Coughland MP (ed) Enzyme systems for lignocellulose degradation. Elsevier, New York, pp 167–181
Ziobro GC (1990a) Origin and nature of kraft color. 1. Role of aromatics. J Wood Chem Technol 10:133–149
Ziobro GC (1990b) Origin and nature of kraft color. 2. The role of bleaching in the formation of the extraction stage effluent color. J Wood Chem Technol 10:151–168
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Patel, R.N., Grabski, A.C. & Jeffries, T.W. Chromophore release from kraft pulp by purified streptomyces roseiscleroticus xylanases. Appl Microbiol Biotechnol 39, 405–412 (1993). https://doi.org/10.1007/BF00192102
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DOI: https://doi.org/10.1007/BF00192102