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Enzymatic hydrolysis of cellulose from oat husks at different substrate concentrations

  • Plant Biopolymers
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Abstract

Pulps prepared from oat husks via a method combining prehydrolysis, alkali delignification, and nitric acid treatment were demonstrated to possess high fermentability upon hydrolysis using multienzyme preparations, such as BrewZyme BGX and CelloLux-A. A dependence of the increment of the yield of reducing substances on the initial substrate concentration ranging from 15 to 120 g/dm3 was studied. The final yield of reducers at 72 h was shown to decline from 88 to 65% with an increase in the initial concentration of the substrate.

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References

  1. Borisenkov, M.V., Shubakov, A.A., Kocheva, L.S., and Karmanov, A.P., Khim. Rastit. Syr’ya, 2011, no. 4, pp. 19–23.

    Google Scholar 

  2. Torlopov, M.A., Tarabukin, D.V., Frolova, S.V., Shcherbakova, T.P., and Volodin, V.V., Khim. Rastit. Syr’ya, 2007, no. 3, pp. 69–76.

    Google Scholar 

  3. Lloyd, T.A. and Wyman, C.E., Bioresource Technol., 2005, vol. 96, pp. 1967–1977.

    Article  CAS  Google Scholar 

  4. Sinitsyn, A.P., Gusakov, A.V., and Chernoglazov, V.M., Biokonversiya lignotsellyuloznykh materialov (Bioconversion of Lignocellulosic Materials), Moscow, 1995.

    Google Scholar 

  5. Silverstein, R.A., Chen, Y., Sharma-Shivappa, R.R., Boyette, M.D., and Osborne, J., Bioresource Technol., 2007, vol. 98, pp. 3000–3011.

    Article  CAS  Google Scholar 

  6. Yoshida, M., Liu, Y., Uchida, S., Kawarada, K., Ukagami, Y., Ichinose, H., Kaneko, S., and Fukuda, K., Biosci. Biotechnol. Biochem., 2008, vol. 72, pp. 805–810.

    Article  CAS  PubMed  Google Scholar 

  7. Yu, Z., Jameel, H., Chang, H.-M., Philips, R., and Park, S., Biotechnol. Bioeng., 2012, vol. 5, pp. 1449–1463.

    Google Scholar 

  8. Taherzaden, M.J. and Karimi, K., BioResources, 2007, vol. 2, no. 4, pp. 707–738.

    Google Scholar 

  9. Obolenskaya, A.V., El’nitskaya, Z.P., and Leonovich, A.A., Laboratornye raboty po khimii drevesiny i tsellyulozy (Laboratory Work on the Chemistry of Wood and Cellulose), Moscow, 1991.

    Google Scholar 

  10. GOST 25438-82. Tsellyuloza dlya khimicheskoi pererabotki. Metody opredeleniya kharakteristicheskoi vyazkosti (GOST 25438-82 10. Cellulose for Chemical Processing: Methods of Determination of Intrinsic Viscosity), Moscow, 1982.

  11. Aleshina, L.A., Lyukhanova, I.V., Budaeva, V.V., Zolotukhin, V.N., Mitrofanov, R.Yu., and Sakovich, G.V., Uchen. Zapiski Petrozavod. Gos. Univ., 2011, no. 8, pp. 114–117.

    Google Scholar 

  12. Gerasimenko, V.L., Laboratornye metody opredeleniya glyukozy: metod. rekomendatsii (Laboratory Methods for Glucose Determination: Guidelines), Izhevsk, 2002.

    Google Scholar 

  13. Veshnyakov, V.A., Khabarov, Yu.G., and Kamakina, N.D., Khim. Rastit. Syr’ya, 2008, no. 4, pp. 47–50.

    Google Scholar 

  14. Makarova, E.I. and Budaeva, V.V., in Tekhnologiya I oborudovanie khimicheskoi, biotekhnologicheskoi i pishchevoi promyshlennosti: mat. 3-i Vseros. nauch.-prakt. konf. studentov, aspirantov i molodykh uchenykh s mezhdunar. uchastiem (Technology and Equipment for Chemical, Biotechnological, and Food Industry: Proc. 3rd All-Russia Scientific and Practical Conference for Students, Graduate Students, and Young Scientists with International Participation.), Biisk, 2010, Part 1, pp. 215–218.

    Google Scholar 

  15. Zolotukhin, V.N. and Budaeva, V.V., in Novye dostizheniya v khimii i khimicheskoi tekhnologii rastitel’nogo syr’ya: mat. V Vseros. konf. (New Advances in Chemistry and Chemical Technology of Plant Raw Materials: Proc. V All-Russia Conference), Barnaul, 2012, pp. 75–77.

    Google Scholar 

  16. Nepenin, N.N., Tekhnologiya tsellyulozy (Cellulose Technology), vol. 1: Proizvodstvo sul’fitnoi tsellyulozy (Production of Sulphite Pulp), Moscow, 1976.

    Google Scholar 

  17. Makarova, E.I., Budaeva, V.V., and Mitrofanov, R.Yu., Polzunovskii Vestnik, 2010, no. 4, pp. 192–198.

    Google Scholar 

  18. Novyi spravochnik khimika i tekhnologa. Syr’e i produkty promyshlennosti organicheskikh i neorganicheskikh veshchestv (A New Handbook of Chemist and Technologist: Raw Materials and Products of Organic and Inorganic Substance Industry), St. Petersburg, 2006.

  19. Felbyu, K., Larsen, Ya., Jorgensen, H., and Vibe-Pedersen, J., EA Patent No. 014759, 2008.

  20. Makarova, E.I., Khim. Interesah Ustoich. Razvit., 2013, vol. 21, no. 2, pp. 219–225.

    CAS  Google Scholar 

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Correspondence to E. I. Makarova.

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Original Russian Text © E.I. Makarova, V.V. Budaeva, E.A. Skiba, 2013, published in Khimiya Rastitel’nogo Syr’ya, 2013, No. 2, pp. 43–50.

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Makarova, E.I., Budaeva, V.V. & Skiba, E.A. Enzymatic hydrolysis of cellulose from oat husks at different substrate concentrations. Russ J Bioorg Chem 40, 726–732 (2014). https://doi.org/10.1134/S1068162014070103

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  • DOI: https://doi.org/10.1134/S1068162014070103

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