Summary
Homozygote plants for the (S) self-incompatibility gene have been produced in Brassica campestris L. var. ‘T 15’. Stigmas from plants designated S 1 S 1, S 2 S 2 and S 4 S 4 were extracted and their protein separated on an isoelectric focusing mini-gel. Differences were observed between proteins from stigmas of the three S-homozygous groups: S-genotype specific proteins were determined for S 1 S 1 and S 2 S 2 stigmas that were absent in the self-compatible S 4 S 4 stigmas. Carbon dioxide (CO2), which is known to block the self-incompatibility reaction in Brassica, was applied to [35S]-methionine unpollinated, self- and cross-pollinated stigmas to observe the effect of external CO2 on the synthesis of these S-associated proteins. The results indicate that pollination triggers a dramatic reduction in protein synthesis in general and in the synthesis of S2-associated protein after self-pollination in particular.
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O'Neill, P.M., Singh, M.B. & Knox, R.B. Biosynthesis of S-associated proteins following self- and cross-pollinations in Brassica campestris L. var. ‘T. 15’. Sexual Plant Reprod 2, 103–108 (1989). https://doi.org/10.1007/BF00191998
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DOI: https://doi.org/10.1007/BF00191998