Abstract
Paramecium bursaria shows a circadian rhythm of photoaccumulation: photoaccumulation is stronger during the day than at night. We obtained five strains of P. bursaria having different circadian periods under continuous light conditions, ranging from 20.9 to 27.9 h. Various physiological activities were compared in the cells of these strains. The periods of contractile vacuole contraction were in the range 10–15 s, which was almost proportional to the periods of the circadian rhythm in each strain. Swimming velocities were inversely proportional to the circadian period; i.e. swimming velocities were high in strains whose circadian periods were short. Resting membrane potential was more depolarized in strains with longer circadian periods. Finally, the membrane resistance of the resting state was reduced in proportion to the increase of the circadian period. Such correlation between the cellular properties and the circadian period suggests that the circadian clock mechanism is associated with various physiological activities of the cell.
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References
Chen TH, Chen TL, Hung LM, Huang TC (1991) Circadian rhythm in amino acid uptake by Cynechococcus RF-1. Plant Physiol 97:55–59
Edmunds LN Jr (1988) Cellular and molecular bases of biological clocks. Springer, New York
Hasegawa K, Tanakadate A (1984) Circadian rhythm of locomotor behavior in a population of Paramecium multimicronucleatum; its characteristics as derived from circadian changes in the swimming speeds and the frequencies of avoiding response among individual cells. Photochem Photobiol 40:105–112
Hiwatashi K (1968) Determination and inheritance of mating type in Paramecium caudatum. Genetics 58:373–386
Johnson CH, Hastings JW (1986) The elusive mechanism of the circadian clock. Am Sci 74:29–36
Johnson CH, Miwa I, Kondo T, Hastings W (1989) Circadian rhythm of photoaccumulation in Paramecium bursaria. J Biol Rhythms 4:405–415
Karakashian MW (1968) The rhythm of mating in Paramecium aurelia, syngen 3. J Cell Physiol 71:197–209
Kitching JA (1967) Contractile vacuoles, ionic regulation and excretion. In: Chen TT (ed) Research in protozoology vol 1. Pergamon Press, New York, pp 307–336
Kondo T, Strayer CA, Kulkarni RD, Taylor W, Ishiura M, Golden SS, Johnson CH (1993) Circadian rhythms in prokaryotes: luciferase as a reporter of circadian gene expression in cyanobacteria. Proc Natl Acad Sci USA 90:5672–5676
Kyriacou CP, Hall J (1980) Circadian rhythm mutations in Drosophila melanogaster affect short-term fluctuations in the male's courtship song. Proc Natl Acad Sci USA 77:6729–6733
Machemer H (1988a) Electrophysiology. In: Gortz HD (ed) Paramecium. Springer, Berlin Heidelberg, pp 185–215
Machemer H (1988b) Motor control of cilia. In: Gortz MD (ed) Paramecium. Springer, Berlin Heidelberg, pp 216–235
Machemer H, Eckert R (1975) Ciliary frequency and orientational responses to clamped voltage steps in Paramecium. J Comp Physiol 104:247–260
Matsuoka K, Nakaoka Y (1988) Photoreceptor potential causing phototaxis of Paramecium bursaria. J Exp Biol 137:477–485
Mergenhagen D (1980) Circadian rhythms in unicellular organisms. Curr Top Microbiol Immunol 90:123–147
Miwa I, Nagatoshi H, Horie T (1987) Circadian rhythmicity within single cell of Paramecium bursaria. J Biol Rhythms 2:57–64
Nakajima K, Nakaoka Y (1989) Circadian change of photosensitivity in Paramecium bursaria. J Exp Biol 144:43–51
Nakaoka Y (1989) Localization of photosensitivity in Paramecium bursaria. J Comp Physiol A 165:637–641
Nakaoka Y, Machemer H (1990) Effects of cyclic nucleotides and intracellular Ca on voltage-activated ciliary beating in Paramecium. J Comp Physiol A 166:401–406
Nakaoka Y, Kinugawa K, Kurotani T (1987) Ca2+-dependent photoreceptor potential in Paramecium bursaria. J Exp Biol 131:107–115
Nakaoka Y, Tokioka R, Shinozawa T, Fujita J, Usukura J (1991) Photoreception of Paramecium cilia: localization of photosensitivity and binding with anti-frog-rhodopsin IgG. J Cell Sci 99:67–72
Oka T, Nakaoka Y, Oosawa F (1986) Changes in membrane potential during adaptation to external potassium ions in Paramecium caudatum. J Exp Biol 126:111–117
Pittendrigh CS (1981) Circadian system: General perspective. In: Aschoff J (ed) Handbook of behavioral neurobiology, vol 4, Biological rhythms. Plenum Press, New York, pp 57–80
Schweiger HG, Hartwig R (1986) Cellular aspects of circadian rhythms J Cell Sci [Suppl] 4:181–200
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Tokushima, H., Okamoto, K.I., Nakaoka, Y. et al. Correlation between circadian periods and cellular activities in Paramecium bursaria . J Comp Physiol A 175, 767–772 (1994). https://doi.org/10.1007/BF00191848
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DOI: https://doi.org/10.1007/BF00191848