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Visual pigment, dark adaptation and rhodopsin renewal in the eye of Pontoporeia affinis (Crustacea, Amphipoda)

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Abstract

The benthic amphipod Pontoporeia affinis lives in the Baltic sea and in northern European lakes in an environment where very little light is available for vision. The eyes, consisting of 40–50 ommatidia, are correspondingly modified. Microspectrophotometric recordings on isolated eyes show the presence of at least two kinds of screening pigments in the ommatidia with maxima at 540–580 nm and 460–500 nm. Difference spectra obtained from the rhabdoms after exposure to red and blue light, respectively, give evidence of a single rhodopsin with its maximum at 548 nm and a 500-nm metarhodopsin. In ERG recordings sensitivity in the dark-adapted state, after saturating exposures to blue and to red light, stabilizes at levels determined by the rhodopsin concentration. No change is observed during 10–14 h after the beginning of dark adaptation. However, using animals pre-exposed with a strong red light and then kept in darkness, it is found that after a delay of 20–40 h sensitivity of the dark-adapted eye begins to increase and finally, after 5–6 days reaches a level corresponding to 100% rhodopsin. Thus, a slow renewal of rhodopsin appears to occur in darkness, where a photoisomerization of metarhodopsin is excluded.

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Abbreviations

ERG:

electroretinogram

IR:

infrared

MSP:

microspectrophotometry

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Authors and Affiliations

  1. Department of Zoology and Tvärminne Zoological Station, University of Helsinki, P.O. Box 17, SF-00014, Helsinki, Finland

    K. O. Donner & M. Lindström

  2. Lehrstuhl für Tierphysiologie, Fakultät für Biologie, Ruhr-Universität Bochum, D-44780, Bochum, Germany

    H. Langer & P. Schlecht

Authors
  1. K. O. Donner
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  2. H. Langer
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  3. M. Lindström
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  4. P. Schlecht
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Donner, K.O., Langer, H., Lindström, M. et al. Visual pigment, dark adaptation and rhodopsin renewal in the eye of Pontoporeia affinis (Crustacea, Amphipoda). J Comp Physiol A 174, 451–459 (1994). https://doi.org/10.1007/BF00191711

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