Skip to main content
SpringerLink
Log in

Detection of transforming gene regions of human papillomavirus type 16 in cervical dysplasias by the polymerase chain reaction

  • Original investigations
  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

In a study of 29 cases of histologically confirmed, characterized colposcopically and cytologically, cervical intraepithelial neoplasias 48.3% (14/ 29) of biopsies were positive for human papillomavirus (HPV) type 16 DNA by polymerase chain reaction. We used two oligonucleotide primer pairs (position 215–514 and 606–805) flanking a 300 and a 199 base pair fragment from the early 6 (E6) and early 7 (E7) genes. The results were concordant both with the E6 and with the E7 regions. Of the amplified products 85.7% (12/14) could be confirmed; these carried 16 specific sequences by Southern blot hybridization. HPV 16 DNA was present in 6.7% (2/30) of the colposcopically directed cytologically normal matched control samples using the same methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Broker TR, Chow LT, Chin MT, Rhodes CR, Wolinsky SM, Whitbeck A, Stoler MH (1989) A nuclear portrait of human papillomavirus carcinogenesis. Cancer cell 7:197–208

    Google Scholar 

  2. Czeglédy J, Gergely L, Hernádi Z, Póka R (1989) Detection of human papillomavirus deoxyribonucleic acid in the female genital tract. Med Microbiol Immunol 178:309–314

    Google Scholar 

  3. Evander M, Wadell G (1990) Oligonucleotide primers for DNA amplification of the early regions 1, 6 and 7 from human papillomavirus types 6, 16, 18, 33. Arch Virol (in press)

  4. Franquemont DW, Ward BE, Andersen WA, Crum CP (1989) Prediction of high-risk cervical papillomavirus infection by biopsy morphology. Am J Clin Pathol 92:577–582

    Google Scholar 

  5. Higuchi R (1989) Rapid efficient DNA extraction for PCR from cells or blood. Amplifications. A forum for PCR users. Perkin Elmer Cetus 2:1–3

    Google Scholar 

  6. Howley PM, Schlegel R (1988) The human papillomavirus. Am J Med 85 [suppl 2A]: 155–158

    Google Scholar 

  7. Johnson MA, Blomfield PI, Bevan IS, Woodman CBJ, Young LS (1990) Analysis of human papillomavirus type 16 E6-E7 transcription in cervical carcinomas and normal cervical epithelium using the polymerase chain reaction. J Gen Virol 71:1473–1479

    Google Scholar 

  8. Lorincz AT, Temple GF, Kurman RJ, Jenson AB, Lancaster WD (1987) Oncogenic association of specific human papillomavirus types with cervical neoplasia. J Natl Cancer Inst 79:671–677

    Google Scholar 

  9. Manos MM, Ting Y, Wright DK, Lewis AJ, Broker TR, Wolinsky SM (1989) Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer cells 7:209–214

    Google Scholar 

  10. Meanwell CA, Cox MF, Blackledge G, Maitland NJ (1987) HPV 16 DNA in normal and malignant cervical epithelium: implications for the aetiology and behaviour of cervical neoplasia. Lancet I:703–707

    Google Scholar 

  11. Melchers WJG, van den Brule A, Walboomers J, de Bruin M, Burger M, Herbrink P, Meijer C, Lindeman J, Quint WGV (1989) Increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified FISH and Southern blot analysis. J Med Virol 27:329–335

    Google Scholar 

  12. Munoz N, Bosch X, Kaldor JM (1988) Does human papillomavirus cause cervical cancer? The state of the epidemiological evidence. Br J Cancer 57:1–5

    Google Scholar 

  13. Pater MM, Pater A (1985) Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145:313–318

    Google Scholar 

  14. Pao CC, Lin CY, Maa JS, Lai CH, Wu SY, Soong YK (1990) Detection of human papillomaviruses in cervicovaginal cells using polymerase chain reaction. J Infect Dis 161:113–115

    Google Scholar 

  15. Pfister H (1987) Human papillomaviruses and genital cancer. Adv Cancer Res 48:113–147

    Google Scholar 

  16. Richart RM (1987) Causes and management of cervical intraepithelial neoplasia. Cancer 60:1951–1991

    Google Scholar 

  17. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354

    Google Scholar 

  18. Shirasawa H, Tomita Y, Kubota K, Kasai T, Sekinya S, Takamizawa H, Simuzu B (1988) Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas. J Virol 62:1022–1027

    Google Scholar 

  19. Van der Brule AJC, Claas ECJ, du Maine M, Melchers WJG, Heimerhorst T, Quint WGV, Lindeman J, Meijer CJLM, Walboomers JMM (1989) Use of anticontamination primers in the polymerase chain reaction for the detection of human papillomavirus genotypes in cervical scrapes and biopsies. J Med Virol 29:20–27

    Google Scholar 

  20. Young LS, Bevan IS, Johnson MA, Blomfield PI, Bromidge T, Maitland NJ, Woodman CBJ (1989) The polymerase chain reaction: a new epidemilogical tool for investigating cervical human papillomavirus infection. Br Med J 298:14–18

    Google Scholar 

  21. Yutsudo M, Okamoto Y, Hakura A (1988) Functional dissociation for transforming genes of human papillomavirus type 16. Virology 166:594–597

    Google Scholar 

  22. zur Hausen H (1986) Intracellular surveillance of persisting viral infections. Human genital cancer results from deficient cellular control of papillomavirus gene expression. Lancet II:489–491

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, University Medical School of Debrecen, P.O. Box 17, H-4012, Debrecen, Hungary

    J. Czeglédy & L. Gergely

  2. Department of Virology, University of Umeå, Sweden

    M. Evander & G. Wadell

  3. Department of Obstetrics and Gynecology, Dr. Kenézy Gyula Hospital, H-4012, Debrecen, Hungary

    L. Veres

Authors
  1. J. Czeglédy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Evander
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Veres
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Gergely
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Wadell
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Czeglédy, J., Evander, M., Veres, L. et al. Detection of transforming gene regions of human papillomavirus type 16 in cervical dysplasias by the polymerase chain reaction. Med Microbiol Immunol 180, 37–43 (1991). https://doi.org/10.1007/BF00191699

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00191699

Keywords

Search

Navigation