, Volume 3, Issue 2, pp 78–84 | Cite as

Studies on ectomycorrhiza

XXII. Mycorrhizal rhizomorphs of Telephora terrestris and Pisolithus tinctorius in association with Norway spruce (Picea abies): formation in vitro and translocation of phosphate
  • H. Kammerbauer
  • R. Agerer
  • H. SandermannJr.
Original Articles


Plants of Picea abies (L.) Karst were grown in mycorrhizal association with Telephora terrestris (Pers. ex Fr.) and Pisolithus tinctorius (Mich. ex Pers.) Coker and Couch on sphagnum peat in petri dishes or Perspex chambers. After 1 year T. terrestris had formed prominent rhizomorphs which were characterized by light microscopy and investigated for 32P-orthophosphate uptake. The absorbed phosphate was transported to sinks throughout the rhizomorphal system as well as into the plant. The calculated translocation velocity and flux rate in the rhizomorph were in the range of 1–3 cm/h and 0.5–4.0 × 10-10 mol cm-2 s-1, respectively. Label was observed to accumulate in the needles 2–3 days after application. Feeding a non-mycorrhized root with 32P-orthophosphate led to an accumulation of label in needles within 1 h, but no radioactivity appeared in the associated T. terrestris rhizomorphs. The rhizomorphs of P. tinctorius revealed a higher structural differentiation than those of T. terrestris. Translocation of labelled phosphorus through rhizomorphs of P. tinctorius into spruce needles was also demonstrated.

Key words

Picea abies Pisolithus tinctorius Phosphate uptake Rhizomorph function Telephora terrestris Translocation 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • H. Kammerbauer
    • 1
    • 2
  • R. Agerer
    • 1
  • H. SandermannJr.
    • 2
  1. 1.Institut für Systematische Botanik der UniversitätMunichFederal Republic of Germany
  2. 2.Institut für Biochemische Pflanzenpathologie, GSF MünchenNeuherbergFederal Republic of Germany

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