Summary
The influence of glutamate on the GABA-activated Cl- conductance was studied in the slowly adapting stretch-receptor neuron and dactylopodite opener muscle fibre of the crayfish (Astacus astacus) using a two-microelectrode and a three-microelectrode voltage clamp, respectively. Glutamate (0.5–1.0 mM) had no effect on the GABA-activated conductance in either preparation. This indicates that the availability of the inhibitory channels for activation of GABA is not influenced by glutamate. The present results are in sharp contrast to those obtained by Franke et al. (J Comp Physiol A 159:591–609, 1986) in experiments on excised membrane patches, which suggested that glutamate is capable of both activating and desensitizing inhibitory postsynaptic channels in the crayfish opener muscle fibre.
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Abbreviations
- GABA :
-
γ-aminobutyric acid
- GGABA and G pGABA :
-
GABA-gated conductance and peak conductance
- HEPES :
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid
- I :
-
current
- SRN :
-
stretch-receptor neuron
- Vm and Vl :
-
membrane voltage in two- and three-microelectrode voltage clamp, respectively
References
Allen CN, Brady R, Swann J, Hori N, Carpenter DO (1988) N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin. Brain Res 458:147–150
Atwood HL (1976) Organization and synaptic physiology of crustacean neuromuscular systems. Prog Neurobiol 7:291–391
Boistel J, Fatt P (1958) Membrane permeability change during inhibitory transmitter action in crustacean muscle. J Physiol (London) 144:176–191
Coombs JS, Eccles JC, Fatt P (1955) The specific ionic conductances and the ionic movements across the motoneuronal membrane that produce the inhibitory post-synaptic potential. J Physiol (Lond) 130:326–373
Dekin MS (1983) Permeability changes induced by l-glutamate at the crayfish neuromuscular junction. J Physiol (Lond) 341:105–125
Dingledine R, Myers SJ, Nicholas RA (1990) Molecular biology of mammalian amino acid receptors. FASEB J 4:2636–2645
Dudel J (1974) Nonlinear voltage dependence of excitatory synaptic current in crayfish muscle. Pflügers Arch 352:227–241
Eccles JC (1964) The physiology of synapses. Springer, Berlin Heidelberg New York
Erecińska M (1987) The neurotransmitter amino acid transport systems. Biochem Pharmacol 36:3547–3555
Franke Ch, Hatt H, Dudel J (1986) The inhibitory chloride channel activated by glutamate as well as γ-amino-butyric acid (GABA). J Comp Physiol A 159:591–609
Hatt H, Franke C, Dudel J (1988) Calcium dependent gating of the l-glutamate activated, excitatory synaptic channel on crayfish muscle. Pflügers Arch 411:17–26
Kaila K, Pasternack M, Saarikoski J, Voipio J (1989) Influence of GABA-gated bicarbonate conductance on membrane potential, current and intracellular chloride in crayfish muscle fibres. J Physiol (Lond) 416:161–181
Kaila K, Rydqvist B, Pasternack M, Voipio J (1992) Inward current caused by sodium-dependent uptake of GABA in the crayfish stretch-receptor neurone. J Physiol (Lond) (in press)
Kanner I, Schuldiner S (1987) Mechanism of transport and storage of neurotransmitters. CRC Crit Rev Biochem 22:1–38
Kuffler SW, Edwards C (1958) Mechanism of gamma aminobutyric acid (GABA) action and its relation to synaptic inhibition. J Neurophysiol 21:589–610
McCarren M, Alger BE (1987) Papain effects on hippocampal neurons in the slice preparation. Neurosci Lett 78:307–310
Nistri A, Constanti A (1979) Pharmacological characterization of different types of GABA and glutamate receptors in vertebrates and invertebrates. Prog Neurobiol 13:117–235
Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB J 4:1469–1480
Onodera K, Takeuchi A (1975) Ionic mechanism of the excitatory synaptic membrane of the crayfish neuromuscular junction. J Physiol (Lond) 252:295–318
Onodera K, Takeuchi A (1976) Permeability changes produced by l-glutamate at the excitatory post-synaptic membrane of the crayfish muscle. J Physiol (Lond) 255:669–685
Pasternack M, Bountra C, Voipio J, Kaila K (1991) Influence of extracellular and intracellular pH on GABA-gated chloride conductance in crayfish muscle fibres. Neuroscience 47:921–929
Roberts E (1986) GABA: the road to neurotransmitter status. In: Olsen RW, Venter JC (eds) Benzodiazepine/GABA receptors and chloride channels; structural and functional properties. Liss, New York, pp 1–39
Rydqvist B, Zhou J-Y (1989) Potential-dependent potassium currents in the slowly adapting stretch receptor neuron of the crayfish. Acta Physiol Scand 137:409–419
Simmonds MA (1983) Multiple GABA receptors and associated regulatory sites. Trends Neurosci 6:279–281
Takeuchi A (1977) Junctional transmission. I. Postsynaptic mechanisms. In: Kandel ER (ed) Handbook of physiology, I, The nervous system, Vol. I. American Physiological Society, Bethesda, pp 295–327
Takeuchi A, Takeuchi N (1965) Localized action of gamma-aminobutyric acid on the crayfish muscle. J Physiol (Lond) 177:225–238
Takeuchi A, Takeuchi N (1966) A study of the inhibitory action of γ-aminobutyric acid on neuromuscular transmission in the crayfish. J Physiol (Lond) 183:418–432
Takeuchi A, Takeuchi N (1967) Anion permeability of the inhibitory post-synaptic membrane of the crayfish neuromuscular junction. J Physiol (Lond) 191:575–590
Takeuchi A, Takeuchi N (1971) Anion interaction at the inhibitory post-synaptic membrane of the crayfish neuromuscular junction. J Physiol (Lond) 212:337–351
Zufall F, Franke C, Hatt H (1988) Acetylcholine activates a chloride channel as well as glutamate and GABA. J Comp Physiol A 163:609–620
Zufall F, Franke C, Hatt H (1989) The insecticide avermectin B1a activates a chloride channel in crayfish muscle membrane. J Exp Biol 142:191–205
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Pasternack, M., Rydqvist, B. & Kaila, K. GABA-gated anion channels in intact crayfish opener muscle fibres and stretch-receptor neurons are neither activated nor desensitized by glutamate. J Comp Physiol A 170, 521–524 (1992). https://doi.org/10.1007/BF00191467
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DOI: https://doi.org/10.1007/BF00191467