A pilot study on morphological compartmentalization and heterogeneity in the elasmobranch olfactory bulb

Abstract

The olfactory bulb of many elasmobranch fishes is morphologically subdivided into distinct units or sub-bulbs immediately adjacent to the olfactory epithelium. We investigated this morphological feature in two species of shark and one species of ray in order to understand its impact on the arrangement of the primary olfactory projections onto the bulb. Using anterograde tracing methods in vitro (biocytin) as well as in fixed tissue (DiI), we observed a direct segregated projection of the olfactory afferents onto the bulb. Application of tracers to the lateral part of the olfactory epithelium resulted in staining restricted to the this region of the bulb, whereas the same tracers applied to the medial part of the epithelium resulted in staining of the medial olfactory bulb. The sub-bulbs appear to be individual anatomical units that each receive input from the olfactory lamellae. Nissl and myelin staining as well as the Golgi method show that the cytoarchitecture of the sub-bulbs is not substantially different from that of other anamniotes. However, we did note the existence of two types of mitral cell, based on the morphology of their dendritic arborization. Type L cells exhibit a loose dendritic arborization, whereas type T cells are characterized by a dense, bush-like dendritic arborization. Both types of mitral cells lack basal dendrites.