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Development of an improved bottle-system for cultivation of strict anaerobes (methanogens)

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Abstract

A simple and efficient bottle-system developed for growth of obligate anaerobes, particularly methanogens, in liquid culture is described. The system consists of a modified plasma bottle fitted with a T-tube permitting direct optical density measurements and cultivation of cells in large quantities under complete anaerobiosis and elevated pressures.

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Authors and Affiliations

  1. Département de Biochimie et Génétique Moléculaire, Unité de Physiologie Cellulaire, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France

    Lakshmi Bhatnagar, Marc Henriquet & Robert Longin

Authors
  1. Lakshmi Bhatnagar
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  2. Marc Henriquet
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  3. Robert Longin
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Bhatnagar, L., Henriquet, M. & Longin, R. Development of an improved bottle-system for cultivation of strict anaerobes (methanogens). Biotechnol Lett 5, 39–42 (1983). https://doi.org/10.1007/BF00189962

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  • DOI: https://doi.org/10.1007/BF00189962

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