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Metalloproteinase production by rabbit articular cartilage: comparison of the effects of interleukin-1α in vitro and in vivo

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Abstract

To assess the effects of interleukin-1 on intact articular cartilage in vitro, explants from young and adult rabbits were cultured with interleukin-1 and the distributions of the matrix metalloproteinases and tissue inhibitor of metalloproteinases (TIMP-1) were investigated by indirect immunofluorescence microscopy. One to 2-week-old cartilage chondrocytes synthesized collagenase in response to pure or crude interleukin-1 (monocyte conditioned medium), with subarticular cells most responsive. Collagenase synthesis was not stimulated in adult articular chondrocytes when explants were treated with either pure or crude interleukin-1. Stromelysin, gelatinase and TIMP-1 could not be demonstrated within any zone of the cartilage, indicating that their synthesis was not stimulated by either pure or crude interleukin-1. The addition of fibroblast growth factors, either alone or in combination with interleukin-1, did not modify these responses. These results contrast markedly with observations on cultured chondrocyte monolayers, where interleukin-1 treatment induces near co-ordinate expression of metalloproteinases. To assess the effects of interleukin-1 in vivo, it was injected into adult rabbit knee joint spaces and the articular cartilage subsequently analysed for evidence of altered metalloproteinase production by immunocytochemistry. No significant increase in metalloproteinase or TIMP-1 synthesis by chondrocytes was detected, although the cartilage matrix showed a marked loss of toluidine blue metachromasia. We conclude that metalloproteinases are not involved in the rapid loss of proteoglycan from cartilage matrix in these situations.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Strangeways Research Laboratory, Worts' Causeway, CB1 4RN, Cambridge, UK

    R. M. Hembry, M. R. Bagga, J. T. Dingle, P. Page Thomas & J. J. Reynolds

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  1. R. M. Hembry
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  2. M. R. Bagga
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  3. J. T. Dingle
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  4. P. Page Thomas
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  5. J. J. Reynolds
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Hembry, R.M., Bagga, M.R., Dingle, J.T. et al. Metalloproteinase production by rabbit articular cartilage: comparison of the effects of interleukin-1α in vitro and in vivo. Vichows Archiv A Pathol Anat 425, 413–424 (1994). https://doi.org/10.1007/BF00189580

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  • DOI: https://doi.org/10.1007/BF00189580

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