Abstract
The hydrophobicity of Bordetella pertussis was assayed by measuring the ability of cells in suspension to adhere to a polystyrene surface. The quantity of adhered bacteria was measured by the binding of enzyme-conjugated anti B. pertussis antibodies. Hydrophobic adherence of non-pathogenic variant strains was about 20% of that exhibited by pathogenic strains. Hydrophobicity was a stable trait as it did not change with passaging or storage. Assays of a series of characterized stable variants suggested that the Filamentous Hemagglutinin (FHA) is the cell surface moiety responsible for hydrophobic adherence in B. pertussis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beachey EH (1981) Bacterial adherence: Adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J Infect Dis 143:325–345
Collier AM, Peterson LP, Baseman JB (1977) Pathogenesis of infection with Bordetella pertussis in hamster tracheal organ. J Infectious Dis 136 suppl:196–203
Fletcher M and Loeb GI (1979) Influence of substratum characteristics on the attachment of a marine Pseudomonad to solid surfaces. Appl Environ Microbiol 37:67–72
Gerson DF, Scheer D (1980) Cell surface energy, contact angles and phase partition. III. Adhesion of bacterial cells to hydrophobic surfaces. Biochim Biophys Acta 602:506–510
Goldman S, Hanski E, Fish F (1984) Spontaneous phase variation in Bordetella pertussis is a multistep non-random process. EMBO J 3:1353–1356
Goldman S, Hanski E, Fish F (1985) Studies on phase variation of Bordetella pertussis. In: Manclark CR and Hennesen W (eds) Proceedings of the 4th International Symposium on Pertussis. S. Karger, Basel
Holt LB (1972) The pathology and immunology of Bordetella pertussis infection. J Med Microbiol 5:407–424
Iida T, Ajiki Y (1974) Growth characteristics of Bordetella pertussis in the chick trachea organ culture. Jap J Microbiol 18:119–126
Irons LI, MacLennan AP (1979) Substrate specificity and the purification by affinity combination methods the two Bordetella pertussis hemagglutinins. In: Manclark CR and Hill JC (eds) International Symposium on Pertussis, DHEW Publication No. 79–1830, US Government Printing Office, Washington DC, pp 338–349
Kabir S and Showkat A (1983) Characterization of surface properties of Vibrio cholerae. Infec Immun 39:1048–1058
Lacey BW (1960) Antigenic modulation of Bordetella pertussis. J Hyg Camb 58:57–93
Leslie PH, Gardner AD (1931) The phases of Haemophilus pertussis. J Hyg 31:423–434
Manclark CR, Cowell JL (1984) Pertussis. In: Germanier R (ed) Bacterial Vaccines, Acad Press, New York, pp 69–106
Matsumaya T (1977) Resistance of Bordetella pertussis phase I to mucocilliary clearance by rabbit tracheal mucous membrane. J Inf Dis 136:609–616
Nakane P (1980) New developments in tissue enzyme-labeled immunoassays. In: Nakamura RM, Dito WR and Tucker ES (eds) Immunoassays. Clinical laboratory techniques for the 1980's, Alan R. Liss, N.Y., pp 157–169
Redhead K (1985) An assay of Bordetella pertussis adhesion to tissue culture cells. J Med Microbiol 19:99–108
Robinson A, Gorringe AR, Irons LI, Keevil CW (1983) Antigenic modulation of Bordetella pertussis in continuous culture. FEMS Microbiol Letters 19:105–109
Rosenberg (1981) Bacterial adherence to polystyrene: A replica method of screening for bacterial hydrophobicity. Appl Environ Microbiol 42:375–377
Rosenberg M, Perry A, Bayer EA, Gutnick DL, Rosenberg E, Ofek Y (1981) Adherence of Acinetobacter calcoaceticus RAG-1 to human epithelial cells and to hexadecane. Infect Immun 33:29–33
Rosenberg M, Rosenberg E (1981) Role of adherence in growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. J Bact 148:51–57
Rosenberg M, Rotem S, Rosenberg E (1982) Cell surface hydrophobicity of smooth and rough Proteus mirabilis strains as determined by adherence to hydrocarbons. FEMS Microbiol Letters 13:167–169
Sato Y, Izumiya K, Oda M-A, Sato H (1979) Biological significance of Bordetella pertussis fimbriae or hemagglutinin: A possible role of the fimbriae or hemagglutinin for pathogenesis and antibacterial immunity. In: Manclark CR and Hill JC (eds) International Symposium on Pertussis, DHEW Publication No. 79–1830, US Government Printing Office, Washington D. C., pp 51–57
Sato Y, Izumiya K, Sato H, Cowell JL, Manclark CR (1981) Role of antibody tp leukocytosis-promoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis. Infect Immun 31:1223–1231
Weiss AA, Falkow S (1984) Genetic analysis of phase change in Bordetella pertussis. Infect Immun 43:263–269
Westergren G, Olsson J (1983) Hydrophobicity and adherence of oral Streptococci after repeated subculture in vitro. Infect Immun 40:432–435
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fish, F., Navon, Y. & Goldman, S. Hydrophobic adherence and phase variation in Bordetella pertussis . Med Microbiol Immunol 176, 37–46 (1987). https://doi.org/10.1007/BF00189407
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00189407