Abstract
We report on results obtained with a direct immunofluorescence test for subtype-specific identification of influenza virus in detached cells of MDCK cultures after inoculation of 281 clinical specimens from patients with influenzalike disease. Influenza virus antibodies were produced in eggs from immunized hens and labelled with FITC. In 157 cases CPE was found in MDCK cells. A total of 57 cases of influenza A (H3N2), 86 cases of influenza A (H1N1), and 14 cases of influenza B were identified. In 33 cases of influenza A (H1N1) infection with massive CPE guinea pig but not chicken erythrocytes were agglutinated by the cell culture supernatants. The single step immunofluorescence test described proved easy to perform and results were obtained within 1 h after CPE was observed in contrast to the conventional HIT which is very time-consuming.
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References
Altschuh D, Hennache G, Regenmortel van MHV (1984) Determination of IgG and IgM levels in serum by rocket immunoelectrophoreses using yolk antibodies from immunized chickens. J Immunol Meth 69:1–7
Bade H, Stegemann H (1984) Rapid method of extraction of antibodies from hen egg yolk. J Immunol Meth 72:421–426
Bjorvatn B, Wolontis S (1972) The use of immunofluorescence in the laboratory diagnosis of influenza B. Scand J Infect Dis 4:177–182
Breitenfeld PM, Schäfer W (1957) The formation of fowl plague virus antigens in infected cells, as studied with fluorescent antibodies. Virology 4:328–345
Burnet FM, Bull DR (1943) Changes in influenza virus associated with adaptation to passage in chick embryos. Austr J Exp Biol Med Sci 21:55–69
Daisy JA, Lief FS, Friedman HM (1979) Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults. J Clin Microbiol 9:688–692
Döller PC, Döller G, Gerth HJ (1985) Immunofluorescence test with antigen-loaded erythrocytes: detection of influenza virus specific IgG, IgA, and IgM antibodies. Med Microbiol Immunol 173:291–302
Dowdle WA, Kendal AP, Nobel GR (1979) Influenza viruses. In: Lennette EH, Schmidt NJ (eds) Diagnostic procedures for viral, rickettsial and chlamydial infections. 6th ed American Public Health association, Washington, DC, pp 585–609
Frank AL, Taber LH, Wells JM (1983) Individuals infected with two subtypes of influenza A virus in the same season. J Infect Dis 1:120–124
Gamboa ET, Wolf A, Yahr MD, Harter DH, Duffy PE, Barden H, Hsu KC (1974) Influenza virus antigen in postencephalitic parkinsonism brain. Arch Neurol 31:228–232
Gardner PS, Kaye S (1982) Egg globulins in rapid virus diagnosis. J Virol Meth 4:257–262
Hercik L, Prochazka (1969) Schnelldiagnostik der Influenza mit der Methode der indirekten Fluoreszenzfärbung. Zentralbl Bakt 211:309–314
Höpken W, Willers H (1978) Influenza-A-Epidemie durch zwei Subtypen in Niedersachsen im Februar/März 1978. Dtsch Med Wochenz 35:1281–1282
Kendal AP, Joseph JM, Kobayashi G, Nelson D, Reyes CR, Ross MR, Sarandria JL, White R, Woodall DF, Noble GR, Dowdle WR (1979) Laboratory-based surveillance of influenza virus in the United States during winter of 1977–1978. I. Periods of prevalence of H1N1 and H3N2 influenza A strains, their relative rates of isolation in different age groups, and detection of antigenic variants. Am J Epidemiol 110:449–461
Kennet ML, Downie J, White J, Ward BK, Mutton KJ, Irving LG, Birch CJ, Rodger SM (1984) Influenza in Melbourne, 1982. Epidemiology and virology. Med J Aust 141:89–92
Meulen ter V, Love R (1967) Virological, immunochemical, and cytochemical studies of four HeLa cell lines infected with two strains of influenza virus. J Virol 1:626–639
Phillips DJ, Galland GG, Reimer CB, Kendal AP (1982) Evaluation of a solid-phase immunoassay with fluorescein isothiocyanate-conjugated heterogeneous or monoclonal antibodies for identification of virus isolates, with influenza virus as a model. J Clin Microbiol 15:931–937
Polson A, Wechmar von B., Regenmortel van MHV (1980) Isolation of viral IgY antibodies from yolks of immunized hens. Immunol Communie 9:475–493
Pyhälä R, Aho K (1982) Seroepidemiology of H1N1 influenza: The range of the affected populations is widening. Med Biol 60:328–332
Rahman AFMS (1973) Early detection of influenza virus in cell culture by means of immunofluorescence. J Clin Path 26:503–505
Tateno Ebisawa I, Makino M, Takeuchi Y (1966) Rapid diagnosis of influenza B with fluorescent antibody. Japan J Exp Med 36:301–320
Tobita K, Suigiura A, Enomoto C, Furuyama M (1975) Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol 162:9–14
Vieira JGH, Oliveira MAD, Russo EMK, Maciel RMB, Pereira AB (1984) Egg yolk as a source of antibodies for human parathyroid hormone (hPTH) radioimmunoassy. J Immunoassay 5:121–129
World Health Organization (1983) Rapid laboratory viral diagnosis. Bull World Hlth Org 61:41–46
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Dedicated to Professor Rudolf Rott on the occasion of his 60th birthday
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Döller, P.C., Döller, G. & Gerth, HJ. Subtype-specific identification of influenza virus in cell cultures with FITC labelled egg yolk antibodies. Med Microbiol Immunol 176, 27–35 (1987). https://doi.org/10.1007/BF00189406
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DOI: https://doi.org/10.1007/BF00189406