Abstract
Vanadium uptake by whole cells and isolated cell walls of the yeast Saccharomyces cerevisiae was studied. When orthovanadate was added to wild-type S. cerevisiae cells growing in rich medium, growth was inhibited as a function of the VO4 3- concentration and the growth was completely arrested at a concentration of 20 mM of VO4 3- in YEPD. Electron paramagnetic resonance (EPR) spectroscopy was used to obtain structural and dynamic information about the cell-associated paramagnetic vanadyl ion. The presence of EPR signals indicated that vanadate was reduced by whole cells to the vanadyl ion. On the contrary, no EPR signals were detected after interaction of vanadate with isolated cell walls. A ‘mobile’ and an ‘immobile’ species associated in cells with small chelates and with macromolecular sites, respectively, were identified. The value of rotational correlation time τ r indicated the relative motional freedom at the macromolecular site. A strongly ‘immobilized’ vanadyl species bound to polar sites mainly through coulombic attractions was detected after interaction of VO2+ ions with isolated cell walls.
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References
Albanese NF, Chasteen ND. 1978 Origin of the electron paramagnetic resonance line widths in frozen solutions of the oxovanadium (IV) ions. J Phys Chem 82, 910–914.
Berardi E, Meloni MG, Bonomo RP, et al. 1990 Electron paramagnetic resonance study of retention of chromium(III), chromate and dichromate (VI) and copper(II) ions by thermotolerant Hansenula polymorpha. J Chem Soc Faraday Trans 86, 2579–2582.
Brand SG, Hawkins CJ, Parry DL. 1987 Acidity and vanadium coordination in vanadocytes. Inorg Chem 26, 627–629.
Bruno GV, Harrington JK, Eastman MP. 1977 Electron spin resonance line shapes of vanadyl complexes in the slow tumbling region. J Phys Chem 81, 1111–1117.
Butt TR, Ecker DJ. 1987 Yeast metallothionein and applications in biotechnology. Microbiol Rev 51, 351–364.
Campbell I. Duffus JH. 1988 Yeast: A Practical Approach. Oxford: IRL Press.
Chasteen ND. 1981 Vandyl(IV) EPR spin probes. Inorganic and biochemical aspects. In: Berliner L, Reuben J, eds. Biological Magnetic Resonance. Plenum Press: New York, 3, 53–119.
Chasteen ND, Francavilla J. 1976 An electron paramagnetic resonance study of vanadyl(IV)-serum albumin complexes. J Ohys Chem 80, 867–871.
Degani H, Gochin M, Karlish SJD, et al. 1981 Electron paramagnetic resonance studies and insulin-line effects of vanadium in rat adipocytes. Biochemistry 20, 5795–5799.
Dickson FE, Kunesh CJ, McGinnis EL, Petrakis L. 1972 Use of electron spin resonance to characterize the vanadium(IV) sulfur species in petroleum. Anal Chem 44, 978–981.
Frank P, Carlosn RMK, Hodgson KO. 1988 Further investigation of the status of acidity and vanadium in the blood cells of Ascidia ceratodes. Inorg Chem 27, 118–122.
Gadd GM, Stewart A, White C, Mowll JL. 1984 Copper uptake by whole cells and protoplasts of wild-type and copper-resistant strain of Saccharomyces cerevisiae. FEMS Microbiol Lett 24, 231–234.
Holyk NH. 1979 An electron paramagnetic resonance study of model oxovanadium(IV) complexes in aqueous solution: correlation of magnetic properties with ligand type and metal chelate structure. MS thesis, University of New Hampshire, Dugham.
Johnson CR, Shepard RE. 1978 Electron spin resonance studies of the solution structure of vanadyl amino acid complexes and mixed ligand complexes of oxalate. Bioinorg Chem 8, 115.
Kanik-Ennulat C, Neff N. 1990 Vanadate-resistant mutants of S. cerevisiae show alterations in protein phosphorylation and growth control. Mol Cell Biol 10, 898–909.
Kinh JC, Mestdagh MM, Rouxhet PG. 1987 ESR study of copper(II) retention by entire cell, cell walls and protoplasts of Saccharomyces cerevisiae. Can J Microbiol 33, 777–782.
Rehder D. 1991 The bioinorganic chemistry of vanadium. Angew Chem Int Ed Engl 30, 148–167.
Ross IS. 1977 Effect of glucose on copper uptake and toxicity in Saccharomyces cerevisiae. Trans Br Mycol Soc 69, 77–81.
Ross IS, Walsh AL. 1981 Resistance to copper in Saccharomyces cerevisae. Trans Br Mycol Soc 77, 27–32.
Schwarz K, Milne DB. 1971 Growth effects of vanadium in the rat. Science 174, 426–428.
Smith TD, Boas JF, Pilbrow JR. 1974 Electron spin resonance study of certain vanadyl polyaminocarboxylate chelates formed in aqueous and frozen aqueous solutions. Aust J Chem 27, 2535–2545.
Thiele DJ. 1992 Metal-regulated transcription in eukaryotes. Nucleic Acids Res 20, 1183–1191.
Toy AD, Chaston SHH, Pilbrow JR. 1971 Electron spin resonance study of the copper(II) chelates of certain monothio-β-diketones and diethyldithiocarbamate. Inorg Chem 10, 2219–2225.
White LK, Chasteen ND. 1979 A Q-band electron paramagnetic resonance study of vanadyl(IV)-labeled human serotransferrin. J Phys Chem 83, 279–284.
van Willigen H, Chandrashekar TK. 1983 ENDOR study of VO2+ adsorbed on Y Zeolite. J Am Chem Soc 105, 4232–4235.
Willsky GR. 1990 Vanadium in the biosphere. In: Chasteen ND, ed. Vanadium in Biological Systems. Kluwer: Dordrecht; 1–24.
Willsky GR, Dosch SF. 1986 Vanadium metabolism in wild type and respiratory-deficient strains of S. cerevisiae. Yeast 2, 77–85.
Willsky GR, Leung JO, Offermann PV, et al. 1985 Isolation and characterization of vanadate-resistant mutants of S. cerevisiae. J Bacteriol 164, 611–617.
Willsky GR, White DA, McCabe BC. 1984 Metabolism of added orthovanadate to vanadyl and high-molecular weight vanadates by Saccharomyces cerevisiae. J Biol Chem 259, 13272–13281.
Zoroddu MA, Berardi E, Fatichenti F. 1989 EPR study of copper(II) retention by thermotolerant Hansenula polymorpha. Yeast 5, S323-S327.
Zoroddu MA, Bonomo RP, Di Bilio AJ, et al. 1991 EPR study on vanadyl and vanadate ion retention by a thermotolerant yeast. J Inorg Biochem 43, 731–738.
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Zoroddu, M.A., Fruianu, M., Dallocchio, R. et al. Electron paramagnetic resonance studies and effects of vanadium in Saccharomyces cerevisiae . Biometals 9, 91–97 (1996). https://doi.org/10.1007/BF00188096
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DOI: https://doi.org/10.1007/BF00188096