Abstract
From the beginning of cardiac myofibrillogenesis in the chick embryo, developing myofibrils at the bottom of the inner myocardial cell layer facing the cardiac jelly are already aligned circumferentially in the direction of the heart tube. To elucidate the mechanism of this alignment, we investigated the temporal and spatial expression of fibronectin and its relationship to actin filaments before and during looping (4- to 13-somite stages) by using a confocal scanning laser microscope. Serial optical tomograms were obtained from whole-mounted heart tubes stained with fluorescein-conjugated antibody against cellular fibronectin and rhodamine-conjugated phalloidin. Before looping (4- to 7-somite stages), particulate and speckled fibronectin formed loose networks. At the onset of looping (8- to 9-somite stages), fine fibrils of fibronectin appeared. They became dense and were arranged circumferentially in the direction of the heart tube. They were aligned parallel with the thick actin bundles that appeared as an initial stage of developing myofibrils. During looping, (10- to 13-somite stages), fibronectin fibrils were fragmented and showed a speckled pattern, while the number of circumferentially aligned mature striated myofibrils increased. These observations suggest that the temporal arrangement of fibronectin fibrils at the beginning of looping plays a role in the circumferential alignment of developing myofibrils.
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References
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (eds) (1989) Cell adhesion, cell junction, and the extracellular matrix. Molecular biology of the cell. Gerland, New York, pp 791–836
Darribere T, Koteliansky VE, Chernovusov MA, Akiyama SK, Yamada KY, Thiery JP, Boucaut JC (1993) Distinct regions of human fibronectin are essential for fibril assembly in an in vivo developing system. Dev Dyn 194:63–70
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Hiruma T, Hirakow R (1985) An ultrastructural topographical study on myofibrillogenesis in the heart of the chick embryo during first onset period. Anat Embryol 172:325–329
Hynes RO (1990) Fibronectins. Springer, New York Berlin Heidelberg, pp 544–546
Hynes RO, Destree AT (1978) Relationships between fibronectin (LETS protein) and actin. Cell 15:875–866
Linask KL, Lash JW (1986) Precardiac cell migration: fibronectin localization at the mesoderm-endoderm interface during directional movement. Dev Biol 114:87–101
Linask KL, Lash JW (1988a) A role of fibronectin in the migration of avian precardiac cells. I. Dose-dependent effects of fibronectin antibody. Dev Biol 129:315–323
Linask KL, Lash JW (1988b) A role of fibronectin in the migration of avian precardiac cells. II. Rotation of the heart-forming region during different stages and its effects. Dev Biol 129:324–329
Peters DM, Mosher DF (1987) Localization of cell surface sites involved in fibronectin fibrillogenesis. J Cell Biol 104:121–130
Shiraishi I, Takamatsu T, Minamikawa T, Fujita S (1992) 3-D observation of actin filament during cardiac looping of the chicken embryonic heart using a confocal laser scanning microscope. Anat Embryol 185:401–408
Shiraishi I, Takamatsu T, Fujita S (1993) 3-D observation of Ncadherin expression during cardiac looping of the chicken embryonic heart using a confocal laser scanning microscope. Anat Embryol 187:115–120
Takamatsu T, Fujita S (1988) Confocal laser scanning microscopy and its three-dimensional application. J Microsc 149:167–174
Yost HJ (1991) Development of the left-right axis in amphibians. In: Bock GR, Marsh J (eds) Biological asymmetry and handedness. Wiley, Chichester, pp 165–181
Yost HJ (1992) Regulation of vertebrate left-right asymmetries by extracellular matrix. Nature 357:158–161
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Shiraishi, I., Takamatsu, T. & Fujita, S. Three-dimensional observation with a confocal scanning laser microscope of fibronectin immunolabeling during cardiac looping in the chick embryo. Anat Embryol 191, 183–189 (1995). https://doi.org/10.1007/BF00187817
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DOI: https://doi.org/10.1007/BF00187817