Abstract
• Background: Two different techniques are available for measurement of macular capillary particle velocities. The psychophysical blue field simulation technique gives data on macular leukocyte flow velocities, while the scanning laser technique provides information on capillary blood velocities of hypofluorescent segments in the macular network. Published velocity data differ considerably between the two methods. The current study was undertaken to compare the two measuring techniques in a group of healthy volunteers. • Methods: Thirty-two healthy subjects (12 man, 20 women, mean age 27 years) participated in this study. All subjects underwent entoptic leukocyte visualization by means of blue field simulation followed by fluorescein angiography using scanning laser ophthalmoscopy. • Results: The capillary blood velocities measured using the scanning laser technique were significantly higher (P < 0.01) than the flow velocities estimated with the blue field simulation technique (2.68 ± 0.3 mm/s vs 0.89 ±0.2 mm/s). No significant correlation between the flow velocities was found (r = −0.22). • Conclusion: The differences may be related to different measuring locations and/or measurements of different phenomena. The blue field technique estimates average leukocyte flow in the macular network, whereas the scanning laser technique quantifies the velocity of erythrocyte aggregates in the capillary lumen of the para- and perifoveal network. A combination of both techniques may be helpful in interpreting physiological responsiveness and altered velocity pattern in diseased eyes.
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Arend O, Wolf S, Jung F, Bertram B, Pöstgens H, Toonen H, Reim M (1991) Retinal microcirculation in patients with diabetes mellitus: dynamic and morphologic analysis of perifoveal capillary network. Br J Ophthalmol 75:514–518
Arend O, Harris A, Wolf S (1994) Capillary blood flow velocity measurements in cystoid macular edema with the scanning laser ophthalmoscope. Am J Ophthalmol 117:819–820
Arend O, Wolf S, Remky A, Sponsel WE, Harris A, Bertram B, Reim M (1994) Perifoveal microcirculation with non-insulin dependent diabetes mellitus. Graefe's Arch Clin Exp Ophthalmol 232:225–231
Bennett AG, Rudnicka AR, Edgar DF (1994) Improvements on Littmann's method of determining the size of retinal features by fundus photography. Graefe's Arch Clin Exp Ophthalmol 232:361–367
Bresnick GH, Condit R, Syrjala S, Palta M, Groo A, Korth K (1984) Abnormalities of the foveal avascular zone in diabetic retinopathy. Arch Ophthalmol 102:1286–93
Chien S, Jan KM (1973) Ultrastructural basis of the mechanism of rouleaux formation. Microvasc Res 5:155–166
Delori FC, Castany MA, Webb RH (1978) Fluorescence characteristics of sodium fluorescein in plasma and whole blood. Exp Eye Res 27:417–425
Fallon TJ, Chowiencyk P, Kohner EM (1986) Measurement of retinal blood flow in diabetes by the blue-light entoptic phenomenon. Br J Ophthalmol 70:43–46
Friedman E, Smith TR, Kuwabara T (1964) Retinal microcirculation in vivo. Invest Opthalmol Vis Sci 3:217–226
Gupta A, Sinclair SH, Sinclair MJ (1993) Non-invasive measurement of the foveal avascular and perifoveal capillary density in normal individuals. Invest Ophthalmol Vis Sci [Suppl] 34:1391
Jung F, Körber N, Kiesewetter H, Prünte C, Wolf S, Reim M (1983) Measuring the microcirculation in the human conjunctiva bulbi under normal and hyperperfusion conditions. Graefes Arch Clin Exp Ophthalmol 220:294–297
Jung F, Kiesewetter H, Wolf S, Pruente C, Körber N, Reim M (1985) Microcirculation of conjunctival capillaries in healthy subjects and in patients with hypertension. Klin Wochenschr 63:1229
Jung F, Wappler M, Nüttgens HP, Kiesewetter H, Wolf S, Muller G (1987) Zur Methodik der Videokapillarmikroskopie: Bestimmung geometrischer und dynamischer Mcβparameter. Biomed Tech 32:204–213
Laatikainen L, Larinkari J (1977) Capillary-free area of the fovea with advancing age. Invest Ophthalmol Vis Sci 16:1154–1157
Littmann H (1988) Zur Bestimmung der wahren Größe eines Objektes auf dem Hintergrund eines lebenden Auges. Klin Monatsbl Augenheilk 192:66–67
Loebl M, Riva CE (1978) Macular circulation and the flying corpuscles phenomenon. Ophthalmology 85:911–917
Marshall C (1935) Entoptic phenomena associated with the retina. Br J Ophthalmol 19:177
Michaelson IC (1948) The mode of development of the retinal vessels and some observations of its significance in certain retinal diseases. Trans Ophthalmol Soc UK 68:137
Michaelson IC, Campbell ACP (1940) The anatomy of the finer retinal vessels, and some observations on their significance in certain retinal diseases. Trans Ophthalmol Soc UK 60:71–112
Nasemann JE, Müller M (1990) Scanning Laser Angiography. In: Naseman JE, Burk ROW (eds) Scanning laser ophthalmoscopy and tomography. Quintessenz, Munich, pp 63–80
Plesch A, Klingbeil U, Bille J (1987) Digital laser scanning fundus camera. Appl Opt 26:1480–1486
Riva CE, Petrig B (1980) Blue field entoptic phenomenon and velocity in the retinal capillaries. J Opt Soc Am 70:1234–1238
Schmid-Schoenbein GW (1987) Rheology of leucocytes. In: Skalak R, Chien S (eds) Handbook of bioengineering. McGraw-Hill, New York, pp 13.1–13.25
Schmid-Sch6nbein H (1986) Rheologie der normalen und pathologischen Blutversorgung der mikroskopischen Conjunktivalgefässe. Fortschr Ophthalmol 83:377–388
Schmid-Schönbein H, Gallasch G, Volger E, Klose HJ (1973) Microrheology and protein chemistry of pathological red cell aggregation (blood sludge) studied in vitro. Biorheology 10:213–227
Sinclair SH (1991) Macular retinal capillary hemodynamics in diabetic patients. Ophthalmology 98:1580–1586
Sinclair SH, Azar-Cavanagh M, Soper KA, Tuma RF, Mayrovitz HN (1989) Investigation of the source of the blue field entoptic phenomenon. Invest Ophthalmol Vis Sci 30:668–673
Sleightholm MA, Arnold J, Kohner EM (1988) Diabetic retinopathy. I. The measurement of intercapillary area in normal retinal angiograms. J Diabetic Complications 2:113–116
Snodderly DM, Weinhaus RS, Choi JC (1992) Neural-vascular relationships in central retina of macaque monkeys (Macaca fascicularis). Neuroscience 12:1169–1193
Sponsel WE, DePaul KL, Kaufman PL (1990) Correlation of visual function and retinal leukocyte velocity in glaucoma. Am J Ophthalmol 109:49–54
Webb RH, Hughes GW, Delori FC (1987) Confocal scanning laser ophthalmoscope. Appl Opt 26:1492–1499
Wolf S, Toonen H, Arend O, Jung F, Kaupp A, Kiesewetter H, Meyer-Ebrecht D, Reim M (1990) Zur Quantifizierung der retinalen Kapillardurchblutung mit Hilfe des Scanning-Laser-Ophthalmoskops. Biomed Tech 35:131–134
Wolf S, Arend O, Toonen H, Bertram B, Jung F, Reim M (1991) Retinal capillary blood flow measurement with a scanning laser ophthalmoscope. Preliminary results. Ophthalmology 98:996–1000
Wolf S, Arend O, Reim M (1994) Measurement of retinal hemodynamics with scanning laser ophthalmoscopy: reference values and variation. Surv Ophthalmol [Suppl] 38:95–100
Wolf S, Arend O, Schulte K, Ittel T, Reim M (1994) Quantification of retinal capillary density and flow velocity in patients with essential hypertension. Hypertension 23:464–467
Yap MKH, Brown B (1994) The repeatability of the noninvasive blue field entoptic phenomenon method for measuring macular capillary blood flow. Optom Vis Sci 71:346–349
Yap M, Gilchrist J, Weatherill J (1987) Psychophysical measurement of the foveal avascular zone. Ophthalmic Physiol Opt 7:405–410
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Arend, O., Harris, A., Sponsel, W.E. et al. Macular capillary particle velocities: a blue field and scanning laser comparison. Graefe's Arch Clin Exp Ophthalmol 233, 244–249 (1995). https://doi.org/10.1007/BF00183599
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DOI: https://doi.org/10.1007/BF00183599