Summary
This study was undertaken to clarify the effects of topically injected chymopapain on the spinal cord and peripheral nerves in rabbits, and also the effect of ganglioside on sciatic nerves which had been damaged by chymopapain. There were no abnormal macroscopic or microscopic findings when chymopapain was injected around the sciatic nerve sheath or around the spinal dura mater, but when it was injected directly into the nerve sheath there was immediate paralysis and severe axonal necrosis; the Schwann sheath remained intact and there was haemorrhage. Injection into the dura resulted in massive haemorrhage, perivascular neutrophil infiltration and necrosis of grey matter. In those treated with ganglioside, regeneration of the damaged sciatic nerve was shorter than in those not so treated.
Résumé
Cette étude a été entreprise pour clarifier les effets de la chymopapaïne injectée localement sur la moëlle épinière et les nerfs périphériques du lapin et ceux de la ganglioside sur les nerfs sciatiques endommagés par la chymopapaïne. Aucun aspect anormal macro — ou microscopique n'est observé quand la chymopapaïne est injectée autour de la gaine du nerf sciatique ou autour de la duremère. Par contre si le produit est injecté directement dans la gaine du nerf la paralysie est immédiate et on observe une grave nécrose des axones. La gaine de Schwann reste intacte et des hémorragies se produisent. L'injection à l'intérieur de la dure-mère entraîne une hémorragie massive, une infiltration périvasculaire de polynucléaires neutrophiles et une nécrose des faisceaux gris. Dans les cas traités par ganglioside la régénération du nerf sciatique est plus lente à 6 semaines que dans ceux qui ne sont pas traités.
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Moon, MS., Kim, I., Ok, IY. et al. The response of nerve tissue to chymopapain. International Orthopaedics 14, 79–83 (1990). https://doi.org/10.1007/BF00183370
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DOI: https://doi.org/10.1007/BF00183370