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Polyamine metabolism in gliomas

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Summary

Biosynthesis of the polyamines putrescine, spermidine, and spermine has been found to be activated in tissues with cellular proliferation. In the present study we have investigated polyamine levels and the activity of the first rate-limiting enzyme ornithine decarboxylase (ODC) in tumour samples obtained during operation of 202 patients with gliomas. Biochemical data were closely related to the grading of malignancy and to the morphological characteristics of each sample. Mean ODC activity was significantly higher in all gliomas as compared to peritumoural non-neoplastic brain. Furthermore, it was significantly higher (p ≤ 0.001) in anaplastic gliomas who grade III and IV (9.0 ± 9.6 nmol/g/h) than in gliomas WHO grade I and II (3.3 ± 4.2 nmol/g/h). Highest enzyme activity (58.5 nmol/g/h) was found in solid and vital parts of malignant tumours, whereas predominantly necrotic areas exhibited low ODC activity (< 1 nmol/g/h). Thus, intra- and interindividual variability of ODC activity corresponded well to histomorphological heterogeneity in high-grade gliomas. Putrescine levels also increased with rising grade of malignancy, whereas spermidine and spermine levels did not correlate with the histological grading. In conclusion, high ODC activity represents a biochemical marker of malignancy in gliomas, but low values do not prove benignity. The present study reinforces the need of further and more extensive tumour sampling closely related to follow-up investigations in the heterogeneous group of gliomas.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Cologne, Cologne, Germany

    Ralf-Ingo Ernestus, Gabriele Röhn, Jin-Yul Lee & Norfrid Klug

  2. Institute for Pathology, Laboratory of Neuropathology, University of Cologne, Cologne, Germany

    Roland Schröder

  3. Department of Experimental Neurology, Max-Planck-Institute for Neurological Research, Cologne, Germany

    Thomas Els & Wulf Paschen

Authors
  1. Ralf-Ingo Ernestus
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  2. Gabriele Röhn
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  3. Roland Schröder
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  4. Thomas Els
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  5. Jin-Yul Lee
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  6. Norfrid Klug
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  7. Wulf Paschen
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Ernestus, RI., Röhn, G., Schröder, R. et al. Polyamine metabolism in gliomas. J Neuro-Oncol 29, 167–174 (1996). https://doi.org/10.1007/BF00182140

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