Abstract
Root organ cultures of red raspberry (Rubus ideaus cv. Titan) were established from two cm long terminal segments of adventitious roots induced in vitro on micropropagated shoot cultures. Growth of root organ cultures was almost entirely by initiation and elongation of lateral roots rather than by terminal elongation from the apical meristem of the original root explants. Indolebutyric acid (IBA) was required for lateral root initiation and elongation. The optimal IBA concentration for lateral root initiation decreased from 0.5 to 0.1 mg/L from the first to the fifth passage in culture. Two cm root explants initiated more lateral roots per unit length than four cm explants. Liquid Anderson's medium was superior to other basal nutrient and vitamin formulations tested. Root growth in liquid culture was stimulated by aeration. Root organ cultures also grew on media solidified with gelrite or agar. A rapid transfer technique was developed for subculturing these which involved cutting and transferring 1 cm discs of roots and underlying medium. Adventitious bud formation occurred spontaneously, but sporadically in liquid cultures, and was not influenced by cytokinin, auxin (spat) light, or chilling.
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References
Anderson WC (1978) Tissue-culture propagation of Rhododendrons. In Vitro 14:334
Anderson WC (1980) Tissue culture propagation or red and black raspberries. Acta Hort 112:13–20
Bachelard EP, Stowe BB (1963) Growth in vitro of roots of Acer rubrum L. and Eucalyptus camaldulensis Dehn. Physiol Plant 16:20–30
Barnes RL, Naylor AW (1959) In vitro culture of pine roots and the use of Pinus serotina roots in metabolic studies. Forest Science 5:158–168
Bonner J (1942) Culture of isolated roots of Acacia melanoxylon. Bull of the Torrey Botanical Club 69(2):130–133
Butcher DN (1980) The culture of isolated roots. In: Ingram DS, Helgeson JP (eds) Tissue culture methods for plant pathologists. Oxford: Blackwell Scientific Publ, pp 13–17
Butcher DN, Street HE (1964) Excised root culture. The Botanical Review 30:513–586
Goforth PL, Torrey JG (1977) The development of isolated roots of Comptonia peregrina (myricaceae) in culture. Amer J Bot 64:476–482
Hackett WP (1983) Phase change and intra-clonal variability. HortScience 18:840
Harada, H (1975) In vitro culture of Actinidia chinensis Pl. as a technique for vegetative multiplication. J Hort Sci 50:81–83
Hartman HT, Kester DE (1983) Plant Propagation. 5th ed Prentice-Hall, Inc, Englewood Cliffs, NJ
Kefford, NP and OH Caso (1972) Organ regeneration on excised roots of Chondrilla juncea and its chemical regulation. Aust J Biol 25:611–706
Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Proc Intl Plant Prop Soc 30:421–427
Momot TS, Arman IA, Szmaylov SF, Smirnov AM, Yatsenko-Khemlevski AA (1974) Amino acid biosynthesis in isolated roots and terminal callus tissues of the European fir (Picea abies (L) Karst). Izv Akad Nauk SSSR Ser Biol 666–671
Mosse B, Hepper C (1975) Vesicular-arbuscular mycorrhizal infections in root organ cultures. Physiol Plant Path 5:215–223
Mukhopadhyay A, Mohanm Ram HY (1981) Regeneration of plantlets from excised roots of Dalbergia sissoo. Indian J Expt Biol 19:1113–1115
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 14:473–497
Rao S, Mohan Ram HY (1981) Regeneration of whole plants from cultured root tips of Limnophila indica. Can J Bot 59:969–973
Rupp LA, Mudge KW (1985) Ethephon and auxin induce mycorrhiza-like changes in the morphology of root organ cultures of Mugo pine. Physiol Plant 64:316–322
Sauton A, Mouras A, Lutz A (1982) Plant regeneration from citrus root meristems. J Hort Sci 57:227–231
Seelinger I (1956) Uber die Kultur isolierter Wurzeln der Robinie (Robinia pseudoacacia L). Flora 144:47–83
Shoemaker JS (1978) Small Fruit Culture. 5th ed AVI Publ Co, Inc, Westport, CT
Slankis V (1973) Hormonal relationships in mycorrhizal development. In: Marks GC, Kozlowski TT (eds) Ectomycorthizae. NY: Academic Press, pp 232–298.
Torrey JG (1958) Endogenous bud and root formation by isolated roots of Convolvulus grown in vitro. Plant Physiol 33:258–263
Ulrich JM (1962) Cultural requirements for growth of excised Ponderosa Pine roots. Physiol Plant 15:59–71
White PR (1934) Potentially unlimited growth of excised tomato root tips in a liquid medium. Plant Physiol 9:585–599
Zelcer A, Soferman O, Izhar S (1983) Shoot regeneration in root cultures of solanaceae. Plant Cell Reports 2:252–254
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Borgman, C.A., Mudge, K.W. Factors affecting the establishment and maintenance of ‘Titan’ red raspberry root organ cultures. Plant Cell Tiss Organ Cult 6, 127–137 (1986). https://doi.org/10.1007/BF00180796
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DOI: https://doi.org/10.1007/BF00180796