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Specific glutathione-SH inhibition of toxic effects of metabolized gentamicin on isolated guinea pig hair cells

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Abstract

The present investigation has shown that only metabolized gentamicin (mG) but not native gentamicin (G) is cytotoxic for isolated guinea pig outer hair cells (OHC). Using FURA-2 fluorescence, both G and mG were found to reduce intracellular free Ca2+ concentrations in unstimulated OHC and inhibit increases in intracellular Ca2+ concentrations during K+-induced depolarization. Glutathione-SH (GSH), a detoxificating agent, did not interfere with G and mG effects on intracellular Ca2+ concentration. In non-stimulated OHC, mG but not G induced pathological OHC depolarization, indicating the opening of transduction channels to allow influx of K+ ions. GSH completely inhibited the lytic effect of mG. Electrophysiological investigations also revealed that GSH probably inhibits mG-induced pathological opening of transduction channels. These results suggest that GSH selectively inhibits mG-specific toxic effects on the guinea pig OHC, possibly by enzymatic detoxification.

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Authors and Affiliations

  1. Department of Otolaryngology, University of Tübingen, Silcherstrasse 5, D-72076, Tübingen, Germany

    H. P. Zenner, S. Keiner & U. Zimmermann

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  1. H. P. Zenner
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  2. S. Keiner
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  3. U. Zimmermann
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Zenner, H.P., Keiner, S. & Zimmermann, U. Specific glutathione-SH inhibition of toxic effects of metabolized gentamicin on isolated guinea pig hair cells. Eur Arch Otorhinolaryngol 251, 84–90 (1994). https://doi.org/10.1007/BF00179898

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  • DOI: https://doi.org/10.1007/BF00179898

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