Abstract
For the evaluation of functional inner ear lesions caused by an autoimmune-induced labyrinthitis, transiently evoked otoacoustic emissions (TEOAEs) were detected in guinea pigs before and after transfer of sensitized lymphocytes from animals suffering from a labyrinthitis induced by the foreign protein keyhole limpet hemocyanin (KLH). Initially TEOAEs were detectable from 47 of 62 ears (76%) in healthy guinea pigs. These animals then were used as recipients for sensitized lymphocytes from donors exclusively. Three months after cell transfer the incidence of TEOAEs was reduced to 48% in animals receiving lymphocytes from donors sensitized with KLH intradermally and intracochlearly. In recipients of lymphocytes from donors sensitized only intradermally and in untreated animals no significant alteration of the TEOAE incidence was found. These findings showed strong correlation with those from scanning electron microscopy of the organ of Corti, indicating that the measurement of TEOAE is a useful, time-saving tool for the detection of cochlear dysfunction caused by sensitized lymphocytes in experimental animal. The present findings also show that the migration of sensitized lymphocytes actually leads to functional lesions in the cochlea.
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References
Avan P, Loth D, Menguy C, Teyssou M (1990) Evoked otoacoustic emissions in the guinea pig: basic characteristics. Hear Res 44:151–160
Bray PJ (1989) Click evoked otoacoustic emissions and the development of a clinical otoacoustic hearing test instrument. PhD Thesis, University of London
Bray P, Kemp D (1987) An advanced cochlear echo technique suitable for infant screening. Br J Audiol 21: 191–204
Gloddek B, Ryan AF, Harris JP (1991) Homing of lymphocytes to the inner ear. Acta Otolaryngol (Stockh) 111: 1051–1059
Harris JP, Low NC, House WF (1985) Contralateral hearing loss following inner ear injury: sympathetic cochleolabyrinthitis? Am J Otol 5:371–377
Kemp DT (1978) Stimulated acoustic emissions from within the human auditory system. J Acoust Soc Am 64:1386–1391
Lehnhardt E (1958) Plötzliche Hörstörungen, auf beiden Seiten gleichzeitig oder nacheinander aufgetreten. Laryngol Rhinol Otol 37:1–5
Malick LE, Wilson RB (1975) Evaluation of a modified technique for SEM examination of vertebrate specimens without evaporated metal layers. In: Jobari O (ed) Scanning electron microscopy, vol 1. ITT Research Institute, Chicago, pp 259–266
Probst R (1990) Otoacoustic emissions: an overview. In: Pfaltz CR (ed) New aspects of inner ear pathophysiology. Advances in Otolaryngology, vol 44. Karger, Basel, pp 1–91
Reiss G (1992) Atypical cells in the normal guinea pig organ of Corti as revealed by micromanipulation in SEM. Microsc Res Tech 20:288–297
Schmiedt RA, Adams JC (1981) Stimulated acoustic emissions in the ear canal of the gerbil. Hear Res 5:295–305
Schreiner L, Wrba H (1961) Zur Frage einer offenen Verbindung zwischen den Perilymphräumen der Innenohren beider Seiten. Klin Wochenschr 39:651–652
Takahashi M, Harris JP (1988) Analysis of immunocompetent cells following inner ear immunostimulation. Laryngoscope 98: 1133–1138
Ueda H, Hattori T, Sawaki M, Niwa H, Yanagita N (1992) The effect of furosemide on evoked otoacoustic emissions in guinea pigs. Hear Res 62:199–205
Wit HP, Ritsma RJ (1980) Evoked acoustical responses from the human ear: some experimental results. Hear Res 2:253–261
Zwicker E, Manley G (1981) Acoustical responses and suppression-period patterns in guinea pigs. Hear Res 4:43–52
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Ross, U.H., Rogowski, M., Reiss, G. et al. Detection of cochlear dysfunction by the measurement of transiently evoked otoacoustic emissions in guinea pigs with autoimmune-induced labyrinthitis. Eur Arch Otorhinolaryngol 251, 80–83 (1994). https://doi.org/10.1007/BF00179897
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DOI: https://doi.org/10.1007/BF00179897