Summary
Two-dimensional versions of HNCA and HNCO experiments are described, which provide essentially the same information as the 3D sequence. A multiple-quantum coherence involving either 15N and 13Cα or 15N and 13CO is created. One of the two frequencies is given by the middle point between the two cross peaks (zero-and double-quantum) and the other by their separation. Quadrature detection can be performed on either nucleus, modifying only the appearence of the 2D spectrum, but not the information content. These experiments, named MQ-HNCA and MQ-HNCO, are illustrated on a (15N, 13C) doubly labelled cytochrome c2 from Rhodobacter capsulatus (116 amino acids).
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Abbreviations
- HMQC:
-
heteronuclear multiple-quantum coherence spectroscopy
- HSQC:
-
heteronuclear single-quantum coherence spectroscopy
- ZQ:
-
zero quantum
- 2Q:
-
double quantum
- MQ:
-
multiple quantum
- TPPI:
-
time-proportional phase increment
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Simorre, JP., Brutscher, B., Caffrey, M.S. et al. Assignment of NMR spectra of proteins using triple-resonance two-dimensional experiments. J Biomol NMR 4, 325–333 (1994). https://doi.org/10.1007/BF00179343
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DOI: https://doi.org/10.1007/BF00179343