Summary
TMV F-IV, isolated from the venom of Trimeresurus mucrosquamatus (TMV), caused rat hindpaw edema in a dose-dependent manner. The maximum hind-paw swelling was reached at 1:5—2 h after subplantar injection of TMV F-IV. The edematous response caused by TMV F-IV was suppressed by the s.c. pretreatment with diphenhydramine, methysergide, acetylsalicylic acid or dexamethasone, and by the subplantar co-injection with FPL 55712, a SRS-A antagonist, and BN 52021 or L 652731, both PAF antagonists. Polymorphonuclear (PMN) leukocyte infiltration appeared within 1 h and gradually increased in the rat paw 3–6 h after edema induction. Compound 48/80 or methotrexate pretreatment also inhibited paw edema caused by TMV F-IV. In isolated mast cells, TMV F-IV increased the formation of PGE2 and LTB4 and caused a dose-dependent release of histamine and β-glucuronidase. Since there are no significant differences in paw edema and mast cell degranulation responses between TMV F-IV and its DFP-modified analogue, the esterase activity may not be necessary in these models. These results indicate that mast cells, PMN leukocytes and some inflammatory mediators such as histamine, serotonin, arachidonate metabolites and PAF are involved in TMV F-IV induced paw edema.
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References
Augstein J, Farmer JB, Lee TB, Sheard P, Tattersald ML (1973) Selective inhibitor of slow reacting substance of anaphylaxis. Nature 245:215–217
Barrett AJ (1972) Lysosomes. In: Dingle JT (ed) A laboratory handbook Elsevier, Amsterdam, pp 118–120
Braquet P, Godfroid JJ (1986) PAF-acether specific binding sites: 2. Design of specific antagonists. Trends Pharmacol Sci 7:397–403
Chen IJ, Chiu HF, Huang HT, Teng CM (1984) Edema formation and degranulation of mast cells by Trimeresurus mucrosquamatus snake venom. Toxicon 22:17–28
Cohen IJ, Zur M, Kaminsky E, De Vries A (1970) Isolation and characterization of kinin-releasing enzyme of Echis coloratus venom. Biochem Pharmacol 19:785–793
Church MK, Miller P (1978) Time course of the anti-anaphylactic and anti-inflammatory effects of dexamethasone in the rat and mouse. Br J Pharmacol Chemother 62:481–486
Daeron M, Sterk AR, Hirata F, Ishizaka T (1982) Biochemical analysis of glucocorticoid-induced inhibition of IgE-mediated histamine release from mouse mast cells. J Immunol 129:1212–1218
Di Rosa M, Giroud JP, Willoughby DA (1971) Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and terpentine. J Pathol 104:15–29
Dri P, Cramer R, Soranzo MR, Comin A, Miotti V, Patriarca P (1982) New approaches to detection of myeloperoxidase deficiency. Blood 60:323–327
Fearn HJ, Smith C, West GB (1964) Capillary permeability responses to snake venom. J Pharm Pharmacol 16:79–84
Håkanson R, Ronnberg AL (1974) Improved fluorometric assay of histamine. Anal Biochem 60:560–567
Hanakae THP (1984) Mechanism of histamine release from rat isolated peritoneal mast cells by dextran: the role of immunoglobulin E. Agents Actions 14:468–474
Higgs GA, Moncada S, Vane JR (1980) The mode of action of anti-inflammatory drugs which prevent the peroxidation of arachidonic acid. Clin Rheum Dis 6:675–693
Holtz P, Raudonat HW, Contzen C (1960) The bradykinin-releasing principle of snake venom. Naunyn-Schmiedeberg's Arch Exp Pathol Pharmacol 239:54–67
Humphrey DM, McManus LM, Satouchi K, Hanahan DJ, Pinckard RN (1982) Vasoactive properties of acetylglyceryl ether phosphorylcholine and analogoues. Lab Invest 46:422–427
Hwang SB, Lam MH, Biflu T, Beattie TR, Shen TY (1985) Trans-2,5-bis-(3,4,5-trimethoxyphenyl)tetrahydrofuran: an orally active specific and competitive receptor antagonist of platelet-activating factor (PAF). J Biol Chem 260:15639–15645
Hwang SB, Lam MH, Li CL, Shen TY (1986) Release of platelet activating factor and its involvement in the first phase of carrageenan-induced rat foot edema. Eur J Pharmacol 120:33–41
Kingston HP, Greaves MW (1985) Actions of prostaglandin E2 metabolites on skin microcirculation. Agents Actions 16:13–14
Kini RM, Evans HJ (1989) A model to explain the pharmacological effects of snake venom phospholipase A2. Toxicon 27:613–635
Kurashige S, Hara Y, Kawakami M, Mitsubashi S (1966) Habu (Trimeresurus flavoviridis) snake venom: VII. Heat stable myolytic factor and development of its activity on addition of phospholipase A2. Jpn J Microbiol 10:23
Majno G, Palade GE (1961) Studies on inflammation: I. The effect of histamine and serotonin on vascular permeability. J Biophys Biochem Cytol 11:571–605
McClain DE, Donlon MA, Chock S, Catravas GN (1983) The effect of calmodulin on histamine release in the rat peritoneal mast cell. Biochim Biophys Acta 736:419–425
Ohsaka A, Takahashi T, Omori-Satoh T, Murata R (1971) Purification and characterization of the hemorrhagic principles in the venom of Trimeresurus flavoviridis. In: De Vries A, Kochva E (eds) Toxins of animal and plant origin. Gordon and Breach, London, p 369
Ohtani Y, Takahashi H (1983) Purification of a capillary permeability increasing-enzyme from the venom of Agkistrodon caliginosus (Kankoku-mamushi). Toxicon 21:871–878
Ouyang C, Shiau SY (1970) Relationship between pharmacological action and enzymatic activities of the venom of Trimeresurus gramineus. Toxicon 8:183–191
Pirotzky E, Page CP, Roubin R, Pflster A, Paul W, Bonnet J, Benveniste J (1984) PAF-acether-induced plasma exudation in rat skin is independent of platelets and neutrophils. Microcir Endothelium Lymphatics 1:107–122
Reid HA (1964) Cobra bites. Br Med J 2:540–545
Rosenberg P (1986) The relationship between enzymatic activity and pharmacological properties of phospholipases in natural poisons. In: Harris JB (ed) Natural toxins. Oxford University Press, Oxford, pp 129–174
Rosenberg P, Condrea E, Rapuano BE, Soons KR, Yang CC (1983) Dissociation of pharmacological and enzymatic activities of snake venom phospholipase A2 by modification of carboxylate groups. Biochem Pharmacol 32:3525–3530
Shore PA, Burkhlter A, Cohn VH Jr (1959) A method for the fluorometric assay of histamine in tissue. J Pharmacol Exp Ther 127:182–186
Sjoerdsma A, Wualkes TP, Weissbach H (1957) Serotonin and histamine in mast cells. Science 125:1202–1203
Tannenbaum S, Oertel H, Henderson W, Kaliner M (1980) The biologic activity of mast cell granules: I. Elicitation of inflammatory responses in rat skin. J Immunol 125:325–335
Teng CM, Wang JP, Peng HC, Ouyang C (1989) Edema-producing proteins isolated from Trimeresurus mucrosquamatus snake venom. Toxicon 27:899–905
Vinegar R, Truax TF, Selph JL (1976) Quantitative studies of the pathway to acute carrageenan inflammation. Fed Proc 35:2447–2456
Vishwanath BS, Kini RM, Gowda TV (1987) Characterization of three edema-inducing phospholipase A2 enzyme from Habu (Trimeresurus flavoviridis) venom and their interaction with the alkaloid aristolochic acid. Toxicon 25:501–515
Wang JP, Teng CM (1988) Rat paw edema induced by trimucase II, a kinin-releasing enzyme from Trimeresurus mucrosquamatus venom. Eur J Pharmacol 157:61–66
Wang JP, Teng CM (1989) Roles of mast cells and PMN leukocytes in cardiotoxin induced rat paw edema. Eur J Pharmacol 161:9–18
Wang JP, Teng CM (1990) Comparison of the enzymatic and edema-producing activities of phospholipase A2 from Naja naja atra and Trimeresurus mucrosquamatus venoms. Eur J Pharmacol 190:347–354
Willoughby DA, Giroud JP (1969) The role of polymorphonuclear leukocyte in acute inflammation in agranulocytic rats. J Pathol (Lond) 98:53–60
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Wang, JP., Peng, HC. & Teng, CM. Rat hind-paw swelling effect of an edema-producing protein isolated from Trimeresurus mucrosquamatus snake venom. Naunyn-Schmiedeberg's Arch Pharmacol 343, 399–404 (1991). https://doi.org/10.1007/BF00179045
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DOI: https://doi.org/10.1007/BF00179045