Summary
The presence and distribution of adrenergic nerves in the developing calvarium of the newborn rat documented by means of the formaldehyde-induced fluorescence technique in rats aged 2 or 7 days. Nerve fibres exhibiting catecholamine-specific fluorescence were seen within the developing calvarium of all animals. In coronal sections, these fibres could be seen in the developing bone, especially in the lamina interna, while in sagittal sections, they were seen to traverse the tissue to reach the central of the diploë. These fibres originate from a denser plexus within the dura mater. Especially in the younger age group, the fluorescent fibres often exhibited an immature appearance, being coarse and devoid of varicosities. In the older animals the fibres were often varicose. The sutural tissue proper was always found to be devoid of adrenergic innervation. The possible origin and functional significance of the adrenergic innervation in the developing bone in relation to skull growth and sutural closure are discussed.
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References
Alberius P, Friede H (1990) Skull growth. In: Hall BK (ed) Bone, vol 6. Telford Press (in press)
Amenta F, Sancesario G, Ferrante F, Cavalotti C (1980) Acetylcholinesterase-containing nerve fibers in the dura mater of guinea-pig, mouse, and rat. J Neural Transm 47:237–242
Andres KH, During M von, Muszynski K, Schmidt RF (1987) Nerve fibres and their terminals of the dura encephali of the rat. Anat Embryol 175:289–301
Bjurholm A, Kreicbergs A, Terenius L, Goldstein M, Schultzberg M (1988) Neuropeptide Y-, tyrosine hydroxylase- and vasoactive intestinal polypeptide-immunoreactive nerves in bone and surrounding tissues. J Auton Nerv Syst 25:119–125
Björklund A (1983) Aldehyde induced catecholamine fluorescence. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, vol 1. Elsevier, Amsterdam, pp 72–190
Clark SL (1931) Innervation of the pia mater of the spinal cord and medulla. J Comp Neurol 53:129–145
Cowen T, Burnstock G (1986) Development, aging, and plasticity of perivascular autonomic nerves. In: Goodman PM (ed) Developmental neurobiology of the autonomic nervous system. Humana Press, New Jersey, pp 211–232
David DJ, Posvillo D, Simpson D (1982) The craniosynostoses. Causes, natural history, and management. Springer, Berlin Heidelberg New York
De Champlain J, Malmfors T, Olsen L, Sachs C (1970) Ontogenesis of peripheral adrenergic neurons in the rat: pre- and postnatal observations. Acta Physiol Scand 80:276–288
Decker JD, Hall S (1985) Light and electron microscopy of the newborn sagittal suture. Anat Rec 212:81–89
Giordano Lanza G, Fusroli P, Bratina F (1972) Sulla innervazione della pachimeninge encefalica. Quad Anat Prat 28:143–154
Gray H (1975) Anatomy of the human body. Goss CM (ed), 29th American edn. Lea & Febiger, Philadelphia
Hill CE, Ngu ME (1987) Development of the extrinsic sympathetic innervation to the enteric neurones of the rat small intestine. J Auton Nerv Syst 19:85–93
Katz DM, Adler JE, Black JB (1986) Catecholaminergic primary sensory neurons. Fed Proc 46:24–30
Keller JT, Marfurt CF, Dimlich RVW, Tierney BE (1989) Sympathetic innervation of the supratentorial dura mater of the rat. J Comp Neurol 290:310–321
Lorén I, Björklund A, Falck B, Lindvall O (1980) The aluminiumformaldehyde (ALFA) histofluorescence method for improved visualization of catecholamines and indoleamines. 1.A detailed account of the methodology for central nervous tissue using paraffin, cryostat or vibratome sections. J Neurosci Methods 2:277–300
Mayberg MR, Zervas NT, Moskowitz MA (1984) Trigeminal projections to supratentorial pial and dural blood vessels in cats demonstrated by horseradish peroidase histochemistry. J Comp Neurol 223:46–56
Moore KL (1982) Clinically oriented anatomy. Williams & Wilkins, Baltimore London
Moss ML (1954) Growth of the calvaria in the rat. The determination of osseous morphology. Am J Anat 94:333–362
Moss ML (1958) Fusion of the frontal suture in the rat. Am J Anat 102:141–160
Olson L, Seiger Å (1980) A system of atypical cetacholamine-containing nerve fibres in the rat iris present after total superior ganglionectomy. Med Biol 48:94–100
Price J, Mudge AW (1983) A subpopulation of rat dorsal root ganglion neurons is catecholaminergic. Nature 301:241–243
Vinkka H (1982) Secondary cartilage in the facial skeleton of the rat. Proc Finn Dent Soc 78 [Suppl VII]:1–137
Xue Z-G, Smith J (1988) High-affinity uptake of noradrenaline in quail dorsal root ganglion cells that express tyrosine hydroxylase immunoreactivity in vitro. J Neurosci 8:806–813
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Alberius, P., Skagerberg, G. Adrenergic innervation of the calvarium of the neonatal rat. Anat Embryol 182, 493–498 (1990). https://doi.org/10.1007/BF00178915
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DOI: https://doi.org/10.1007/BF00178915